Gene description for Cd81
Gene name CD81 antigen
Gene symbol Cd81
Other names/aliases Tapa-1
Tapa1
Tspan28
Species Mus musculus
 Database cross references - Cd81
ExoCarta ExoCarta_12520
Entrez Gene 12520
UniProt P35762  
 Cd81 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Microglia 16081791    
Oligodendrocytes 21136642    
 Gene ontology annotations for Cd81
Molecular Function
    MHC class II protein complex binding GO:0023026 ISO
    protein binding GO:0005515 IPI
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 ISO
    positive regulation of 1-phosphatidylinositol 4-kinase activity GO:0043128 ISO
    positive regulation of B cell proliferation GO:0030890 TAS
    protein localization GO:0008104 ISO
    regulation of cell proliferation GO:0042127 ISO
    regulation of cell motility GO:2000145 IGI
    positive regulation of cell growth GO:0030307 TAS
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    phosphatidylinositol metabolic process GO:0046488 ISO
    activation of MAPK activity GO:0000187 ISO
    positive regulation of cell proliferation GO:0008284 ISO
Subcellular Localization
    apical plasma membrane GO:0016324 ISO
    immunological synapse GO:0001772 ISO
    integral component of plasma membrane GO:0005887 ISO
    integral component of membrane GO:0016021 ISO
    vesicle GO:0031982 ISO
    membrane GO:0016020 ISO
    focal adhesion GO:0005925 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Cd81 in exosomes
1
Experiment ID 73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 14
ISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11
EV Cytosolic markers
✔
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
4
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Cd81
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cd81 is involved
PathwayEvidenceSource
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell IEA Reactome





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