Gene description for Cd81
Gene name CD81 antigen
Gene symbol Cd81
Other names/aliases Tapa-1
Tapa1
Tspan28
Species Mus musculus
 Database cross references - Cd81
ExoCarta ExoCarta_12520
Vesiclepedia VP_12520
Entrez Gene 12520
UniProt P35762  
 Cd81 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 30918259    
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Faeces 33431899    
Faeces 33431899    
Fibroblasts 23260141    
Kidney medullary cells 34580290    
Kidney medullary cells 34580290    
Kidney medullary cells 34580290    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Microglia 16081791    
Oligodendrocytes 21136642    
Plasma 32132711    
Plasma 32132711    
Plasma 32132711    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cd81
Molecular Function
    virus receptor activity GO:0001618 IEA
    virus receptor activity GO:0001618 ISO
    integrin binding GO:0005178 ISO
    integrin binding GO:0005178 ISS
    protein binding GO:0005515 IPI
    cholesterol binding GO:0015485 ISO
    cholesterol binding GO:0015485 ISS
    MHC class II protein binding GO:0042289 IEA
    MHC class II protein binding GO:0042289 ISO
    transferrin receptor binding GO:1990459 IEA
    transferrin receptor binding GO:1990459 ISO
Biological Process
    immunological synapse formation GO:0001771 ISO
    immunological synapse formation GO:0001771 ISS
    humoral immune response mediated by circulating immunoglobulin GO:0002455 ISO
    humoral immune response mediated by circulating immunoglobulin GO:0002455 ISS
    positive regulation of inflammatory response to antigenic stimulus GO:0002863 IMP
    myoblast fusion involved in skeletal muscle regeneration GO:0014905 IMP
    positive regulation of B cell proliferation GO:0030890 ISO
    positive regulation of B cell proliferation GO:0030890 TAS
    receptor internalization GO:0031623 IDA
    regulation of protein stability GO:0031647 IEA
    regulation of protein stability GO:0031647 ISO
    macrophage fusion GO:0034238 IMP
    macrophage fusion GO:0034238 ISO
    CD4-positive, alpha-beta T cell costimulation GO:0035783 ISO
    CD4-positive, alpha-beta T cell costimulation GO:0035783 ISS
    regulation of cell population proliferation GO:0042127 ISO
    positive regulation of MAPK cascade GO:0043410 ISO
    positive regulation of MAPK cascade GO:0043410 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    symbiont entry into host cell GO:0046718 IEA
    positive regulation of B cell receptor signaling pathway GO:0050861 IMP
    positive regulation of B cell receptor signaling pathway GO:0050861 ISO
    positive regulation of T cell receptor signaling pathway GO:0050862 ISO
    positive regulation of T cell receptor signaling pathway GO:0050862 ISS
    positive regulation of B cell activation GO:0050871 IMP
    protein localization to lysosome GO:0061462 IEA
    protein localization to lysosome GO:0061462 ISO
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 ISO
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 ISS
    cellular response to low-density lipoprotein particle stimulus GO:0071404 IDA
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to plasma membrane GO:0072659 ISS
    osteoclast fusion GO:0072675 IMP
    positive regulation of receptor clustering GO:1903911 IMP
    positive regulation of receptor clustering GO:1903911 ISO
    positive regulation of receptor clustering GO:1903911 ISS
    positive regulation of protein catabolic process in the vacuole GO:1904352 IEA
    positive regulation of protein catabolic process in the vacuole GO:1904352 ISO
    regulation of macrophage migration GO:1905521 IDA
    positive regulation of adaptive immune memory response GO:1905676 IEA
    positive regulation of adaptive immune memory response GO:1905676 ISO
    regulation of cell motility GO:2000145 IGI
    positive regulation of T-helper 2 cell cytokine production GO:2000553 IMP
    positive regulation of T-helper 2 cell cytokine production GO:2000553 ISO
    positive regulation of T-helper 2 cell cytokine production GO:2000553 ISS
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 ISO
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 ISS
    positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:2001190 IMP
    positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:2001190 ISO
    positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:2001190 ISS
Subcellular Localization
    immunological synapse GO:0001772 ISO
    immunological synapse GO:0001772 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    basal plasma membrane GO:0009925 ISO
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    vesicle GO:0031982 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    tetraspanin-enriched microdomain GO:0097197 ISO
    tetraspanin-enriched microdomain GO:0097197 ISS
 Experiment description of studies that identified Cd81 in sEVs
1
Experiment ID 944
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Mus musculus
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name 4T1-luci (+)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 73
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
4
Experiment ID 303
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
5
Experiment ID 304
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
6
Experiment ID 210
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 1148
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34580290    
Organism Mus musculus
Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Authors "Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL."
Journal name Nat Commun
Publication year 2021
Sample Kidney medullary cells
Sample name Kidney medullary cells - ciliated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
small RNA sequencing
8
Experiment ID 1149
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34580290    
Organism Mus musculus
Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Authors "Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL."
Journal name Nat Commun
Publication year 2021
Sample Kidney medullary cells
Sample name Kidney medullary cells - ciliated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
small RNA sequencing
9
Experiment ID 1150
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34580290    
Organism Mus musculus
Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Authors "Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL."
Journal name Nat Commun
Publication year 2021
Sample Kidney medullary cells
Sample name Kidney medullary cells - ciliated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
small RNA sequencing
10
Experiment ID 1101
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 1102
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 1103
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
13
Experiment ID 1104
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 1105
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2 - Fraction 5
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
15
Experiment ID 1106
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
16
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 14
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
19
Experiment ID 33
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
20
Experiment ID 333
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 334
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 335
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cd81
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Igsf8 140559
Affinity Capture-MS Mus musculus
2 Ifitm3 66141
Affinity Capture-Western Mus musculus
3 Ifitm2  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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