Gene description for Ifitm3
Gene name interferon induced transmembrane protein 3
Gene symbol Ifitm3
Other names/aliases 1110004C05Rik
Cd225
Cdw217
DSPA2b
Fgls
IP15
mil-1
Species Mus musculus
 Database cross references - Ifitm3
ExoCarta ExoCarta_66141
Vesiclepedia VP_66141
Entrez Gene 66141
UniProt Q9CQW9  
 Ifitm3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ifitm3
Biological Process
    receptor-mediated endocytosis GO:0006898 IMP
    negative regulation of cell population proliferation GO:0008285 IDA
    response to virus GO:0009615 IDA
    response to virus GO:0009615 ISO
    negative regulation of viral transcription GO:0032897 ISO
    response to type II interferon GO:0034341 IBA
    response to type II interferon GO:0034341 ISO
    response to interferon-alpha GO:0035455 IBA
    response to interferon-alpha GO:0035455 ISO
    response to interferon-beta GO:0035456 IBA
    response to interferon-beta GO:0035456 ISO
    negative regulation of viral genome replication GO:0045071 IBA
    negative regulation of viral genome replication GO:0045071 ISO
    negative regulation of viral entry into host cell GO:0046597 IBA
    negative regulation of viral entry into host cell GO:0046597 IDA
    negative regulation of viral entry into host cell GO:0046597 ISO
    defense response to virus GO:0051607 IBA
    defense response to virus GO:0051607 IDA
    defense response to virus GO:0051607 IMP
    defense response to virus GO:0051607 ISO
    defense response to virus GO:0051607 ISO
    type I interferon-mediated signaling pathway GO:0060337 IBA
    type I interferon-mediated signaling pathway GO:0060337 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    endoplasmic reticulum GO:0005783 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IDA
    cytoplasmic vesicle GO:0031410 IDA
    early endosome membrane GO:0031901 ISO
    early endosome membrane GO:0031901 ISS
    late endosome membrane GO:0031902 IEA
    protein-containing complex GO:0032991 ISO
    apical part of cell GO:0045177 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified Ifitm3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ifitm3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cd81 12520
Affinity Capture-Western Mus musculus
2 Atp6v0b  
Affinity Capture-Western Mus musculus
3 Nphs2  
Affinity Capture-MS Mus musculus
4 Usp7  
Affinity Capture-MS Mus musculus
5 Tmem173  
Affinity Capture-Western Mus musculus
Proximity Label-MS Mus musculus
View the network image/svg+xml



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