Gene description for Col18a1
Gene name collagen, type XVIII, alpha 1
Gene symbol Col18a1
Other names/aliases -
Species Mus musculus
 Database cross references - Col18a1
ExoCarta ExoCarta_12822
Vesiclepedia VP_12822
Entrez Gene 12822
UniProt P39061  
 Col18a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Col18a1
Molecular Function
    protein binding GO:0005515 IPI
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    skeletal system development GO:0001501 IBA
    angiogenesis GO:0001525 IBA
    angiogenesis GO:0001525 IDA
    angiogenesis GO:0001525 IMP
    endothelial cell morphogenesis GO:0001886 IBA
    endothelial cell morphogenesis GO:0001886 IDA
    cell adhesion GO:0007155 IEA
    extracellular matrix organization GO:0030198 IBA
    extracellular matrix organization GO:0030198 IMP
    blood vessel endothelial cell migration GO:0043534 IDA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IDA
    endothelial cell apoptotic process GO:0072577 IDA
    vascular endothelial cell proliferation GO:0101023 IDA
    positive regulation of vascular endothelial cell proliferation GO:1905564 IDA
    positive regulation of endothelial cell apoptotic process GO:2000353 IDA
Subcellular Localization
    collagen trimer GO:0005581 IBA
    collagen trimer GO:0005581 IEA
    basement membrane GO:0005604 IDA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
 Experiment description of studies that identified Col18a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Col18a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gpc4  
Co-fractionation Mus musculus
2 Fbln2 14115
Co-fractionation Mus musculus
3 Sell  
Co-fractionation Mus musculus
4 Pou5f1  
Affinity Capture-MS Mus musculus
5 Hspg2 15530
Co-fractionation Mus musculus
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