Gene description for Ctsl
Gene name cathepsin L
Gene symbol Ctsl
Other names/aliases 1190035F06Rik
Ctsl1
MEP
fs
nkt
Species Mus musculus
 Database cross references - Ctsl
ExoCarta ExoCarta_13039
Vesiclepedia VP_13039
Entrez Gene 13039
UniProt P06797  
 Ctsl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ctsl
Molecular Function
    fibronectin binding GO:0001968 ISO
    endopeptidase activity GO:0004175 IMP
    aminopeptidase activity GO:0004177 ISO
    aminopeptidase activity GO:0004177 ISS
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 ISO
    cysteine-type endopeptidase activity GO:0004197 ISS
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 ISO
    cysteine-type peptidase activity GO:0008234 ISO
    cysteine-type carboxypeptidase activity GO:0016807 IDA
    kininogen binding GO:0030984 ISO
    peptide binding GO:0042277 ISO
    peptide binding GO:0042277 ISS
    histone binding GO:0042393 IDA
    histone binding GO:0042393 ISO
    proteoglycan binding GO:0043394 ISO
    protein-containing complex binding GO:0044877 ISO
    metal ion binding GO:0046872 IEA
    serpin family protein binding GO:0097655 ISO
Biological Process
    proteolysis GO:0006508 IDA
    proteolysis GO:0006508 ISO
    proteolysis GO:0006508 ISS
    thyroid hormone generation GO:0006590 IMP
    spermatogenesis GO:0007283 ISS
    male gonad development GO:0008584 ISS
    cellular response to starvation GO:0009267 ISS
    response to glucose GO:0009749 ISS
    negative regulation of keratinocyte proliferation GO:0010839 IMP
    response to organic cyclic compound GO:0014070 ISS
    nerve development GO:0021675 ISS
    protein catabolic process GO:0030163 ISO
    collagen catabolic process GO:0030574 IDA
    hair follicle morphogenesis GO:0031069 IMP
    zymogen activation GO:0031638 ISS
    enkephalin processing GO:0034230 ISS
    response to gonadotropin GO:0034698 ISS
    catagen GO:0042637 IMP
    CD4-positive, alpha-beta T cell lineage commitment GO:0043373 IMP
    keratinocyte proliferation GO:0043616 IMP
    decidualization GO:0046697 IGI
    decidualization GO:0046697 ISS
    antigen processing and presentation of peptide antigen GO:0048002 IMP
    autophagic cell death GO:0048102 ISS
    response to glucocorticoid GO:0051384 ISS
    proteolysis involved in protein catabolic process GO:0051603 IBA
    proteolysis involved in protein catabolic process GO:0051603 IMP
    Sertoli cell differentiation GO:0060008 ISS
    elastin catabolic process GO:0060309 IMP
    epithelial tube branching involved in lung morphogenesis GO:0060441 IMP
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 ISS
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosome GO:0005764 ISO
    multivesicular body GO:0005771 ISO
    Golgi apparatus GO:0005794 ISO
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 ISO
    microvillus GO:0005902 ISS
    external side of plasma membrane GO:0009897 IDA
    external side of plasma membrane GO:0009897 ISO
    external side of plasma membrane GO:0009897 ISS
    apical plasma membrane GO:0016324 IEA
    secretory granule GO:0030141 ISO
    secretory granule GO:0030141 ISS
    cytoplasmic vesicle GO:0031410 IDA
    chromaffin granule GO:0042583 ISS
    perikaryon GO:0043204 ISO
    perikaryon GO:0043204 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    apical part of cell GO:0045177 ISO
    apical part of cell GO:0045177 ISS
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Ctsl in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ctsl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hba-a1 15122
Biochemical Activity Mus musculus
2 Hbb 15127
Biochemical Activity Mus musculus
View the network image/svg+xml



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