Gene description for Ecm1
Gene name extracellular matrix protein 1
Gene symbol Ecm1
Other names/aliases AI663821
p85
Species Mus musculus
 Database cross references - Ecm1
ExoCarta ExoCarta_13601
Vesiclepedia VP_13601
Entrez Gene 13601
UniProt Q61508  
 Ecm1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ecm1
Molecular Function
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    interleukin-2 receptor binding GO:0005134 IPI
    extracellular matrix structural constituent GO:0005201 RCA
    protein binding GO:0005515 IPI
Biological Process
    ossification GO:0001503 IEA
    angiogenesis GO:0001525 IEA
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    positive regulation of endothelial cell proliferation GO:0001938 ISS
    negative regulation of cytokine-mediated signaling pathway GO:0001960 IDA
    chondrocyte development GO:0002063 IMP
    regulation of type 2 immune response GO:0002828 IMP
    endochondral bone growth GO:0003416 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    inflammatory response GO:0006954 IMP
    signal transduction GO:0007165 IEA
    negative regulation of peptidase activity GO:0010466 ISO
    negative regulation of peptidase activity GO:0010466 ISS
    regulation of bone mineralization GO:0030500 IBA
    regulation of bone mineralization GO:0030500 IMP
    negative regulation of bone mineralization GO:0030502 ISO
    negative regulation of bone mineralization GO:0030502 ISS
    biomineral tissue development GO:0031214 IEA
    positive regulation of angiogenesis GO:0045766 ISO
    positive regulation of angiogenesis GO:0045766 ISS
    regulation of T cell migration GO:2000404 IMP
Subcellular Localization
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 ISO
 Experiment description of studies that identified Ecm1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ecm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbln1 14114
Co-fractionation Mus musculus
2 Hspg2 15530
Co-fractionation Mus musculus
View the network image/svg+xml



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