Gene description for Rps3
Gene name ribosomal protein S3
Gene symbol Rps3
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rps3
ExoCarta ExoCarta_140654
Vesiclepedia VP_140654
Entrez Gene 140654
UniProt P62909  
 Rps3 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rps3
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    DNA binding GO:0003677 ISO
    damaged DNA binding GO:0003684 ISO
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IC
    structural constituent of ribosome GO:0003735 IEA
    structural constituent of ribosome GO:0003735 ISO
    DNA-(apurinic or apyrimidinic site) endonuclease activity GO:0003906 IEA
    DNA-(apurinic or apyrimidinic site) endonuclease activity GO:0003906 ISO
    DNA endonuclease activity GO:0004520 ISO
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 ISO
    tubulin binding GO:0015631 ISO
    DNA N-glycosylase activity GO:0019104 ISO
    enzyme binding GO:0019899 ISO
    kinase binding GO:0019900 IPI
    kinase binding GO:0019900 ISO
    protein kinase binding GO:0019901 ISO
    Hsp70 protein binding GO:0030544 ISO
    oxidized purine DNA binding GO:0032357 ISO
    oxidized pyrimidine DNA binding GO:0032358 ISO
    ubiquitin-like protein conjugating enzyme binding GO:0044390 ISO
    protein-containing complex binding GO:0044877 ISO
    protein kinase A binding GO:0051018 ISO
    Hsp90 protein binding GO:0051879 ISO
    small ribosomal subunit rRNA binding GO:0070181 ISO
    supercoiled DNA binding GO:0097100 ISO
    class I DNA-(apurinic or apyrimidinic site) endonuclease activity GO:0140078 IEA
    DNA-binding transcription factor binding GO:0140297 ISO
Biological Process
    DNA repair GO:0006281 ISO
    base-excision repair GO:0006284 ISO
    translation GO:0006412 IC
    translation GO:0006412 IEA
    apoptotic process GO:0006915 IEA
    DNA damage response GO:0006974 IDA
    DNA damage response GO:0006974 ISO
    chromosome segregation GO:0007059 ISO
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of translation GO:0017148 ISO
    positive regulation of microtubule polymerization GO:0031116 ISO
    positive regulation of protein-containing complex assembly GO:0031334 IEA
    positive regulation of protein-containing complex assembly GO:0031334 ISO
    negative regulation of protein ubiquitination GO:0031397 ISO
    positive regulation of endodeoxyribonuclease activity GO:0032079 ISO
    positive regulation of interleukin-2 production GO:0032743 ISO
    cellular response to reactive oxygen species GO:0034614 ISO
    positive regulation of activated T cell proliferation GO:0042104 ISO
    regulation of apoptotic process GO:0042981 IMP
    regulation of apoptotic process GO:0042981 ISO
    positive regulation of JUN kinase activity GO:0043507 ISO
    negative regulation of DNA repair GO:0045738 ISO
    positive regulation of DNA repair GO:0045739 ISO
    positive regulation of T cell receptor signaling pathway GO:0050862 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    spindle assembly GO:0051225 ISO
    cell division GO:0051301 IEA
    response to TNF agonist GO:0061481 ISO
    cellular response to hydrogen peroxide GO:0070301 ISO
    cellular response to tumor necrosis factor GO:0071356 ISO
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISO
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902231 ISO
    positive regulation of base-excision repair GO:1905053 ISO
    cellular response to nerve growth factor stimulus GO:1990090 IDA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    positive regulation of apoptotic signaling pathway GO:2001235 IBA
    positive regulation of apoptotic signaling pathway GO:2001235 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial matrix GO:0005759 ISO
    endoplasmic reticulum GO:0005783 ISO
    spindle GO:0005819 IEA
    cytosol GO:0005829 ISO
    ribosome GO:0005840 IEA
    ribosome GO:0005840 ISO
    plasma membrane GO:0005886 ISO
    postsynaptic density GO:0014069 ISO
    small ribosomal subunit GO:0015935 IEA
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 IEA
    cytosolic small ribosomal subunit GO:0022627 ISO
    dendrite GO:0030425 IDA
    ruffle membrane GO:0032587 ISO
    synapse GO:0045202 IEA
    synapse GO:0045202 ISO
    NF-kappaB complex GO:0071159 ISO
    mitotic spindle GO:0072686 ISO
    cytoplasmic side of rough endoplasmic reticulum membrane GO:0098556 ISO
    ribonucleoprotein complex GO:1990904 IEA
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Rps3 in exosomes
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rps3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Snca  
Affinity Capture-MS Rattus norvegicus
3 Sumo3  
Affinity Capture-MS Rattus norvegicus
4 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
5 Park7 117287
Affinity Capture-MS Rattus norvegicus
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