Gene description for Fasn
Gene name fatty acid synthase
Gene symbol Fasn
Other names/aliases A630082H08Rik
FAS
Species Mus musculus
 Database cross references - Fasn
ExoCarta ExoCarta_14104
Entrez Gene 14104
UniProt P19096  
 Fasn identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Pancreatic cells 19351151    
 Gene ontology annotations for Fasn
Molecular Function
    identical protein binding GO:0042802 ISO
    enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity GO:0047117 IEA
    palmitoyl-[acyl-carrier-protein] hydrolase activity GO:0016296 IEA
    [acyl-carrier-protein] S-acetyltransferase activity GO:0004313 IEA
    enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity GO:0004319 IEA
    zinc ion binding GO:0008270 IEA
    protein homodimerization activity GO:0042803 ISO
    3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity GO:0047451 IEA
    NADPH binding GO:0070402 ISO
    3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity GO:0004316 IEA
    catalytic activity GO:0003824 IEA
    acyl-[acyl-carrier-protein] hydrolase activity GO:0016297 IEA
    3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity GO:0004317 IEA
    drug binding GO:0008144 ISO
    3-oxoacyl-[acyl-carrier-protein] synthase activity GO:0004315 IEA
    oleoyl-[acyl-carrier-protein] hydrolase activity GO:0004320 IEA
    hydrolase activity, acting on ester bonds GO:0016788 IEA
    hydrolase activity GO:0016787 IEA
    transferase activity GO:0016740 IEA
    oxidoreductase activity GO:0016491 IEA
    [acyl-carrier-protein] S-malonyltransferase activity GO:0004314 IEA
    3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity GO:0019171 IEA
    fatty acid synthase activity GO:0004312 ISO
    poly(A) RNA binding GO:0044822 ISO
    myristoyl-[acyl-carrier-protein] hydrolase activity GO:0016295 IEA
    lyase activity GO:0016829 IEA
Biological Process
    biosynthetic process GO:0009058 IEA
    acetyl-CoA metabolic process GO:0006084 ISO
    oxidation-reduction process GO:0055114 IEA
    fatty acid biosynthetic process GO:0006633 ISO
    cellular response to interleukin-4 GO:0071353 IDA
    metabolic process GO:0008152 IEA
    lipid metabolic process GO:0006629 IEA
    osteoblast differentiation GO:0001649 ISO
    fatty acid metabolic process GO:0006631 IEA
Subcellular Localization
    membrane GO:0016020 ISO
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    mitochondrion GO:0005739 IDA
    extracellular exosome GO:0070062 ISO
    glycogen granule GO:0042587 IDA
 Experiment description of studies that identified Fasn in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Fasn
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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