Gene description for Pdia3
Gene name protein disulfide isomerase associated 3
Gene symbol Pdia3
Other names/aliases 58kDa
ERp57
ERp60
ERp61
Erp
Grp58
PDI
PDI-Q2
PI-PLC
PLC[a]
Plca
Species Mus musculus
 Database cross references - Pdia3
ExoCarta ExoCarta_14827
Entrez Gene 14827
UniProt P27773  
 Pdia3 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Pdia3
Molecular Function
    peptidase activity GO:0008233 ISO
    protein-disulfide reductase (glutathione) activity GO:0019153 ISO
    protein disulfide isomerase activity GO:0003756 IBA
    MHC class I protein binding GO:0042288 ISO
    poly(A) RNA binding GO:0044822 ISO
    protein binding GO:0005515 IPI
    isomerase activity GO:0016853 IEA
Biological Process
    response to endoplasmic reticulum stress GO:0034976 IBA
    proteolysis GO:0006508 ISO
    oxidation-reduction process GO:0055114 ISO
    protein folding GO:0006457 IBA
    positive regulation of protein folding GO:1903334 ISO
    cell redox homeostasis GO:0045454 IEA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IMP
    cellular response to vitamin D GO:0071305 ISO
Subcellular Localization
    myelin sheath GO:0043209 IDA
    smooth endoplasmic reticulum GO:0005790 ISO
    extracellular exosome GO:0070062 ISO
    extracellular space GO:0005615 ISO
    endoplasmic reticulum GO:0005783 ISO
    TAP complex GO:0042825 ISO
    extracellular region GO:0005576 ISO
    cell surface GO:0009986 ISO
    focal adhesion GO:0005925 ISO
    mitochondrion GO:0005739 ISO
    plasma membrane GO:0005886 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    acrosomal vesicle GO:0001669 ISO
    apical plasma membrane GO:0016324 ISO
    mitochondrial intermembrane space GO:0005758 ISO
 Experiment description of studies that identified Pdia3 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Pdia3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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