Gene description for CTNS
Gene name cystinosin, lysosomal cystine transporter
Gene symbol CTNS
Other names/aliases CTNS-LSB
PQLC4
Species Homo sapiens
 Database cross references - CTNS
ExoCarta ExoCarta_1497
Vesiclepedia VP_1497
Entrez Gene 1497
HGNC 2518
MIM 606272
UniProt O60931  
 CTNS identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for CTNS
Molecular Function
    L-cystine transmembrane transporter activity GO:0015184 NAS
Biological Process
    long-term memory GO:0007616 IEA
    melanin biosynthetic process GO:0042438 IEA
    ion transport GO:0006811 TAS
    lens development in camera-type eye GO:0002088 IEA
    transmembrane transport GO:0055085 TAS
    grooming behavior GO:0007625 IEA
    glutathione metabolic process GO:0006749 IMP
    adult walking behavior GO:0007628 IEA
    cellular amino acid metabolic process GO:0006520 NAS
    brain development GO:0007420 IMP
    ATP metabolic process GO:0046034 IMP
    visual learning GO:0008542 IEA
    L-cystine transport GO:0015811 NAS
    cognition GO:0050890 IMP
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 IDA
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 IEA
    melanosome GO:0042470 IEA
    intermediate filament cytoskeleton GO:0045111 IDA
    late endosome GO:0005770 IDA
    lysosomal membrane GO:0005765 TAS
    lysosome GO:0005764 IDA
    early endosome GO:0005769 IDA
 Experiment description of studies that identified CTNS in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CTNS
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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