Gene description for CTNS
Gene name cystinosin, lysosomal cystine transporter
Gene symbol CTNS
Other names/aliases CTNS-LSB
PQLC4
Species Homo sapiens
 Database cross references - CTNS
ExoCarta ExoCarta_1497
Vesiclepedia VP_1497
Entrez Gene 1497
HGNC 2518
MIM 606272
UniProt O60931  
 CTNS identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for CTNS
Molecular Function
    protein binding GO:0005515 IPI
    L-cystine transmembrane transporter activity GO:0015184 IBA
    L-cystine transmembrane transporter activity GO:0015184 IDA
    L-cystine transmembrane transporter activity GO:0015184 IMP
    L-cystine transmembrane transporter activity GO:0015184 NAS
    L-cystine transmembrane transporter activity GO:0015184 TAS
    solute:proton symporter activity GO:0015295 IDA
Biological Process
    lens development in camera-type eye GO:0002088 IEA
    amino acid metabolic process GO:0006520 NAS
    glutathione metabolic process GO:0006749 IMP
    monoatomic ion transport GO:0006811 TAS
    brain development GO:0007420 IMP
    long-term memory GO:0007616 IEA
    grooming behavior GO:0007625 IEA
    adult walking behavior GO:0007628 IEA
    visual learning GO:0008542 IEA
    negative regulation of hydrogen peroxide biosynthetic process GO:0010730 IEA
    positive regulation of mitochondrial membrane potential GO:0010918 IEA
    protein transport GO:0015031 IEA
    L-cystine transport GO:0015811 IBA
    L-cystine transport GO:0015811 IDA
    L-cystine transport GO:0015811 IDA
    L-cystine transport GO:0015811 IMP
    L-cystine transport GO:0015811 NAS
    thyroid gland development GO:0030878 IEA
    renal D-glucose absorption GO:0035623 IEA
    melanin biosynthetic process GO:0042438 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    ATP metabolic process GO:0046034 IMP
    regulation of melanin biosynthetic process GO:0048021 IMP
    cognition GO:0050890 IMP
    transmembrane transport GO:0055085 TAS
    renal water absorption GO:0070295 IEA
    proximal tubule morphogenesis GO:0072158 IEA
    renal albumin absorption GO:0097018 IEA
    renal phosphate ion absorption GO:0097291 IEA
    proton transmembrane transport GO:1902600 IEA
    regulation of TORC1 signaling GO:1903432 IDA
    positive regulation of TORC1 signaling GO:1904263 IEA
    brush border assembly GO:1904970 IEA
    positive regulation of thyroid hormone generation GO:2000611 IEA
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 NAS
    lysosomal membrane GO:0005765 TAS
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IEA
    melanosome membrane GO:0033162 IEA
    melanosome GO:0042470 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CTNS in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CTNS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX58 23586
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here