Gene description for Ptpn6
Gene name protein tyrosine phosphatase, non-receptor type 6
Gene symbol Ptpn6
Other names/aliases 70Z-SHP
Hcph
PTPTY-42
Ptp1C
SH-PTP1
SHP-1
hcp
me
Species Mus musculus
 Database cross references - Ptpn6
ExoCarta ExoCarta_15170
Vesiclepedia VP_15170
Entrez Gene 15170
UniProt P29351  
 Ptpn6 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ptpn6
Molecular Function
    transmembrane receptor protein tyrosine phosphatase activity GO:0005001 ISO
    SH3 domain binding GO:0017124 IDA
    phosphoprotein phosphatase activity GO:0004721 IEA
    phosphatase activity GO:0016791 IEA
    hydrolase activity GO:0016787 IEA
    protein kinase binding GO:0019901 ISO
    SH2 domain binding GO:0042169 IDA
    protein tyrosine phosphatase activity GO:0004725 IDA
    protein binding GO:0005515 IPI
    natural killer cell lectin-like receptor binding GO:0046703 ISO
    phosphotyrosine binding GO:0001784 IPI
Biological Process
    cytokine-mediated signaling pathway GO:0019221 TAS
    regulation of B cell differentiation GO:0045577 IMP
    negative regulation of MAP kinase activity GO:0043407 IMP
    peptidyl-tyrosine dephosphorylation GO:0035335 ISO
    negative regulation of B cell receptor signaling pathway GO:0050859 IGI
    regulation of G1/S transition of mitotic cell cycle GO:2000045 ISO
    protein dephosphorylation GO:0006470 ISO
    cell proliferation GO:0008283 ISO
    abortive mitotic cell cycle GO:0033277 IMP
    hematopoietic progenitor cell differentiation GO:0002244 IMP
    regulation of ERK1 and ERK2 cascade GO:0070372 ISO
    natural killer cell mediated cytotoxicity GO:0042267 IMP
    peptidyl-tyrosine phosphorylation GO:0018108 ISO
    negative regulation of T cell receptor signaling pathway GO:0050860 IMP
    positive regulation of cell proliferation GO:0008284 ISO
    positive regulation of cell adhesion mediated by integrin GO:0033630 IMP
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 ISO
    megakaryocyte development GO:0035855 IMP
    dephosphorylation GO:0016311 IEA
    platelet formation GO:0030220 IGI
    negative regulation of T cell proliferation GO:0042130 IMP
    negative regulation of MAPK cascade GO:0043409 IMP
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IGI
    negative regulation of humoral immune response mediated by circulating immunoglobulin GO:0002924 IMP
    platelet aggregation GO:0070527 IMP
    signal transduction GO:0007165 ISO
    intracellular signal transduction GO:0035556 IDA
    cell differentiation GO:0030154 ISO
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 ISO
    B cell receptor signaling pathway GO:0050853 IMP
Subcellular Localization
    cytoplasm GO:0005737 TAS
    nucleus GO:0005634 ISO
    intracellular GO:0005622 IDA
    extracellular exosome GO:0070062 ISO
    nucleolus GO:0005730 ISO
    alpha-beta T cell receptor complex GO:0042105 IDA
    cell-cell junction GO:0005911 IDA
 Experiment description of studies that identified Ptpn6 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ptpn6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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