Gene description for Hells
Gene name helicase, lymphoid specific
Gene symbol Hells
Other names/aliases AI323785
E130115I21Rik
LSH
Lysh
PASG
YFK8
Species Mus musculus
 Database cross references - Hells
ExoCarta ExoCarta_15201
Vesiclepedia VP_15201
Entrez Gene 15201
UniProt Q60848  
 Hells identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Hells
Molecular Function
    chromatin binding GO:0003682 IBA
    chromatin binding GO:0003682 IDA
    helicase activity GO:0004386 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
Biological Process
    urogenital system development GO:0001655 IMP
    kidney development GO:0001822 IMP
    chromatin remodeling GO:0006338 IMP
    DNA methylation-dependent constitutive heterochromatin formation GO:0006346 IBA
    DNA methylation-dependent constitutive heterochromatin formation GO:0006346 IMP
    apoptotic process GO:0006915 IMP
    lymphocyte differentiation GO:0030098 TAS
    heterochromatin formation GO:0031507 IMP
    pericentric heterochromatin formation GO:0031508 IBA
    pericentric heterochromatin formation GO:0031508 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 IBA
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 IMP
    lymphocyte proliferation GO:0046651 IBA
    lymphocyte proliferation GO:0046651 IEP
    cell division GO:0051301 IEA
    chromosomal DNA methylation maintenance following DNA replication GO:0141119 IMP
    cellular response to leukemia inhibitory factor GO:1990830 IEP
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IMP
Subcellular Localization
    chromosome, centromeric region GO:0000775 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    pericentric heterochromatin GO:0005721 IBA
    pericentric heterochromatin GO:0005721 IDA
 Experiment description of studies that identified Hells in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Hells
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mcm7 17220
Co-fractionation Mus musculus
2 Mecom  
Co-fractionation Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Mcm4 17217
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Mms22l  
Co-fractionation Mus musculus
7 Pou5f1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
8 Nanog  
Affinity Capture-MS Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
10 Tfcp2l1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Hells is involved
No pathways found





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