Gene description for Hspa4
Gene name heat shock protein 4
Gene symbol Hspa4
Other names/aliases 70kDa
AI317151
APG-2
Hsp110
Hsp70RY
mKIAA4025
Species Mus musculus
 Database cross references - Hspa4
ExoCarta ExoCarta_15525
Entrez Gene 15525
 Hspa4 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
 Gene ontology annotations for Hspa4
Molecular Function
    activating transcription factor binding GO:0033613 ISO
    RNA polymerase II transcription factor binding GO:0001085 ISO
    protein complex binding GO:0032403 ISO
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
Biological Process
    positive regulation of protein binding GO:0032092 ISO
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    protein import into mitochondrial outer membrane GO:0045040 ISO
    chaperone-mediated protein complex assembly GO:0051131 ISO
    negative regulation of cell death GO:0060548 ISO
    negative regulation of DNA binding GO:0043392 ISO
    negative regulation of protein phosphorylation GO:0001933 ISO
    response to heat GO:0009408 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    lipid particle GO:0005811 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Hspa4 in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Hspa4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hspa4 is involved
No pathways found





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