Gene description for Ilk
Gene name integrin linked kinase
Gene symbol Ilk
Other names/aliases AA511515
ESTM24
Species Mus musculus
 Database cross references - Ilk
ExoCarta ExoCarta_16202
Vesiclepedia VP_16202
Entrez Gene 16202
UniProt O55222  
 Ilk identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ilk
Molecular Function
    protein binding GO:0005515 IPI
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    protein kinase activity GO:0004672 ISO
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activity GO:0004674 ISO
    SH3 domain binding GO:0017124 ISO
    kinase activity GO:0016301 IEA
    transferase activity GO:0016740 IEA
    signal transducer activity GO:0004871 ISO
    protein kinase binding GO:0019901 ISO
    nucleotide binding GO:0000166 IEA
    integrin binding GO:0005178 ISO
Biological Process
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of protein kinase activity GO:0006469 ISO
    regulation of signal transduction GO:0009966 IEA
    negative regulation of neural precursor cell proliferation GO:2000178 IMP
    peptidyl-serine phosphorylation GO:0018105 ISO
    cell projection organization GO:0030030 IMP
    positive regulation of MAPK cascade GO:0043410 ISO
    positive regulation of cell-matrix adhesion GO:0001954 ISO
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    Schwann cell development GO:0014044 IMP
    cell cycle arrest GO:0007050 ISO
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 ISO
    positive regulation of phosphorylation GO:0042327 ISO
    positive regulation of myoblast differentiation GO:0045663 ISO
    positive regulation of transcription, DNA-templated GO:0045893 ISO
    fibroblast migration GO:0010761 IMP
    protein phosphorylation GO:0006468 ISO
    neuron projection morphogenesis GO:0048812 ISO
    positive regulation of cell proliferation GO:0008284 ISO
    positive regulation of protein kinase B signaling GO:0051897 ISO
    cell aging GO:0007569 ISO
    protein kinase B signaling GO:0043491 IMP
    myelination in peripheral nervous system GO:0022011 IMP
    positive regulation of BMP signaling pathway GO:0030513 IMP
    positive regulation of cell migration GO:0030335 ISO
    phosphorylation GO:0016310 IEA
    platelet aggregation GO:0070527 ISO
    negative regulation of neuron apoptotic process GO:0043524 ISO
    extracellular fibril organization GO:0043206 ISO
    nerve development GO:0021675 IMP
    positive regulation of dendrite morphogenesis GO:0050775 ISO
    myelin assembly GO:0032288 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    regulation of cell growth GO:0001558 ISO
    positive regulation of axon extension GO:0045773 ISO
    positive regulation of osteoblast differentiation GO:0045669 IMP
    negative regulation of smooth muscle cell proliferation GO:0048662 ISO
    branching involved in ureteric bud morphogenesis GO:0001658 IDA
    positive regulation of MAP kinase activity GO:0043406 ISO
    negative regulation of smooth muscle cell migration GO:0014912 ISO
    regulation of actin cytoskeleton organization GO:0032956 ISO
    protein heterooligomerization GO:0051291 ISO
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IMP
    integrin-mediated signaling pathway GO:0007229 ISO
    positive regulation of axonogenesis GO:0050772 ISO
Subcellular Localization
    cell projection GO:0042995 IEA
    cell-cell junction GO:0005911 ISO
    lamellipodium GO:0030027 IDA
    neuronal cell body GO:0043025 ISO
    protein complex GO:0043234 ISO
    dendritic shaft GO:0043198 ISO
    stress fiber GO:0001725 ISO
    focal adhesion GO:0005925 ISO
    cell junction GO:0030054 ISO
    axon GO:0030424 ISO
    nucleoplasm GO:0005654 ISO
    plasma membrane GO:0005886 IEA
    terminal bouton GO:0043195 ISO
    costamere GO:0043034 ISO
    cytoplasm GO:0005737 ISO
    intracellular GO:0005622 IMP
    membrane GO:0016020 ISO
    extracellular matrix GO:0031012 ISO
 Experiment description of studies that identified Ilk in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ilk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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