Gene description for Ilk
Gene name integrin linked kinase
Gene symbol Ilk
Other names/aliases AA511515
ESTM24
Species Mus musculus
 Database cross references - Ilk
ExoCarta ExoCarta_16202
Vesiclepedia VP_16202
Entrez Gene 16202
UniProt O55222  
 Ilk identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ilk
Molecular Function
    magnesium ion binding GO:0000287 ISS
    protein kinase activity GO:0004672 ISO
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    signaling receptor binding GO:0005102 IBA
    integrin binding GO:0005178 ISO
    integrin binding GO:0005178 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISS
    SH3 domain binding GO:0017124 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein domain specific binding GO:0019904 ISO
    protein-macromolecule adaptor activity GO:0030674 IBA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    cell morphogenesis GO:0000902 IEA
    cell morphogenesis GO:0000902 ISO
    regulation of cell growth GO:0001558 ISO
    branching involved in ureteric bud morphogenesis GO:0001658 IDA
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of cell-matrix adhesion GO:0001954 ISO
    outflow tract morphogenesis GO:0003151 IMP
    protein phosphorylation GO:0006468 ISO
    mitotic spindle organization GO:0007052 IEA
    cell-matrix adhesion GO:0007160 IBA
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 ISO
    integrin-mediated signaling pathway GO:0007229 ISO
    cell population proliferation GO:0008283 IMP
    positive regulation of cell population proliferation GO:0008284 IMP
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of signal transduction GO:0009967 ISO
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 ISO
    fibroblast migration GO:0010761 IMP
    Schwann cell development GO:0014044 IMP
    negative regulation of smooth muscle cell migration GO:0014912 ISO
    peptidyl-serine phosphorylation GO:0018105 IMP
    nerve development GO:0021675 IMP
    myelination in peripheral nervous system GO:0022011 IMP
    cell projection organization GO:0030030 IMP
    cell differentiation GO:0030154 IBA
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of BMP signaling pathway GO:0030513 IMP
    myelin assembly GO:0032288 ISO
    regulation of actin cytoskeleton organization GO:0032956 ISO
    tumor necrosis factor-mediated signaling pathway GO:0033209 IEA
    tumor necrosis factor-mediated signaling pathway GO:0033209 ISO
    substrate adhesion-dependent cell spreading GO:0034446 IBA
    substrate adhesion-dependent cell spreading GO:0034446 IEA
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    positive regulation of phosphorylation GO:0042327 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    positive regulation of MAPK cascade GO:0043410 ISO
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IMP
    positive regulation of myoblast differentiation GO:0045663 ISO
    positive regulation of osteoblast differentiation GO:0045669 IMP
    positive regulation of axon extension GO:0045773 ISO
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    negative regulation of smooth muscle cell proliferation GO:0048662 ISO
    neuron projection morphogenesis GO:0048812 ISO
    positive regulation of axonogenesis GO:0050772 ISO
    positive regulation of dendrite morphogenesis GO:0050775 ISO
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    neural precursor cell proliferation GO:0061351 IGI
    neural precursor cell proliferation GO:0061351 IMP
    caveola assembly GO:0070836 IMP
    protein localization to cell cortex GO:0072697 IMP
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    supramolecular fiber organization GO:0097435 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    negative regulation of neural precursor cell proliferation GO:2000178 IGI
    negative regulation of neural precursor cell proliferation GO:2000178 IMP
    positive regulation of cellular senescence GO:2000774 ISO
Subcellular Localization
    chromatin GO:0000785 ISS
    stress fiber GO:0001725 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    cell-cell junction GO:0005911 ISO
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 IDA
    focal adhesion GO:0005925 ISO
    cell cortex GO:0005938 IEA
    sarcomere GO:0030017 IEA
    lamellipodium GO:0030027 IDA
    axon GO:0030424 ISO
    protein-containing complex GO:0032991 ISO
    neuronal cell body GO:0043025 ISO
    costamere GO:0043034 ISO
    terminal bouton GO:0043195 ISO
    dendritic shaft GO:0043198 ISO
 Experiment description of studies that identified Ilk in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ilk
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sdf4  
Co-fractionation Mus musculus
2 Mbp 17196
Biochemical Activity Mus musculus
3 Fanci  
Co-fractionation Mus musculus
4 Parvb 170736
Co-fractionation Mus musculus
5 Lims1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
6 Atxn1  
Proximity Label-MS Mus musculus
7 Lpcat1  
Co-fractionation Mus musculus
8 Haus5  
Co-fractionation Mus musculus
9 Tecr  
Co-fractionation Mus musculus
10 Parva  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 Tmsb4x  
Affinity Capture-Western Mus musculus
12 Pou5f1  
Affinity Capture-MS Mus musculus
13 Fancd2  
Affinity Capture-MS Mus musculus
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