Experiment description of studies that identified Itgb2 in exosomes

1 Experiment ID 10 ISEV standards ✔ EM EV Biophysical techniques ✔ Alix EV Cytosolic markers ✔ CD9|MHCII|MHCI|CD86|LAMP2 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] PubMed ID 11390481     Organism Mus musculus Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S. Journal name JIMMU Publication year 2001 Sample Dendritic cells Sample name D1 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting

2 Experiment ID 214 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name Leishmania-infected-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 215 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name LPS-treated-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 216 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name Normal-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 5 ISEV standards ✔ EM|IEM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD86|CD80 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method ELISA PubMed ID 11145662     Organism Mus musculus Experiment description Mast cell-dependent B and T lymphocyte activation is mediated by the secretion of immunologically active exosomes. Authors Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, David B, Namane A, Mecheri S Journal name JIMMU Publication year 2001 Sample Mast cells Sample name P815 MC9 Bone marrow-derived mast cells Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF] ELISA

6 Experiment ID 6 ISEV standards EV Biophysical techniques EV Cytosolic markers EV Membrane markers EV Negative markers EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 11306949     Organism Mus musculus Experiment description Nonspecific B and T cell-stimulatory activity mediated by mast cells is associated with exosomes. Authors Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, Namane A, David B, Mecheri S Journal name IAAI Publication year 2001 Sample Mast cells Sample name Bone marrow-derived mast cells Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

7 Experiment ID 14 ISEV standards ✔ EM EV Biophysical techniques ✔ RAB7|RAB11 EV Cytosolic markers ✔ CD9|CD63|LAMP1|LAMP2 EV Membrane markers ✔ DNM EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 16081791     Organism Mus musculus Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L Journal name JIMMU Publication year 2005 Sample Microglia Sample name N9 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA XP] Western blotting

Protein-protein interactions for Itgb2

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Itgb2 is involved

No pathways found