Gene description for Itgb2
Gene name integrin beta 2
Gene symbol Itgb2
Other names/aliases 2E6
AI528527
Cd18
LAD
LCAMB
Lfa1
MF17
Species Mus musculus
 Database cross references - Itgb2
ExoCarta ExoCarta_16414
Vesiclepedia VP_16414
Entrez Gene 16414
UniProt P11835  
 Itgb2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 11145662    
Mast cells 11306949    
Microglia 16081791    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Itgb2
Molecular Function
    receptor activity GO:0004872 IEA
    glycoprotein binding GO:0001948 ISO
    protein heterodimerization activity GO:0046982 ISO
    metal ion binding GO:0046872 IEA
    protein complex binding GO:0032403 ISO
    ICAM-3 receptor activity GO:0030369 ISO
    protein binding GO:0005515 IPI
    cell adhesion molecule binding GO:0050839 ISO
    protein kinase binding GO:0019901 ISO
Biological Process
    natural killer cell activation GO:0030101 ISO
    neutrophil chemotaxis GO:0030593 ISO
    cellular extravasation GO:0045123 ISO
    signal transduction GO:0007165 ISO
    receptor clustering GO:0043113 ISO
    endothelial cell migration GO:0043542 ISO
    integrin-mediated signaling pathway GO:0007229 IDA
    leukocyte migration involved in inflammatory response GO:0002523 ISO
    activated T cell proliferation GO:0050798 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
    cell-matrix adhesion GO:0007160 ISO
    single organismal cell-cell adhesion GO:0016337 ISO
    positive regulation of angiogenesis GO:0045766 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 ISO
    heterotypic cell-cell adhesion GO:0034113 ISO
    cell adhesion GO:0007155 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
Subcellular Localization
    plasma membrane GO:0005886 ISO
    extracellular vesicle GO:1903561 ISO
    membrane raft GO:0045121 IDA
    receptor complex GO:0043235 ISO
    integrin alphaL-beta2 complex GO:0034687 ISO
    cell surface GO:0009986 ISO
    integrin complex GO:0008305 IEA
    integral component of membrane GO:0016021 IEA
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Itgb2 in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 10
MISEV standards
EM
EV Biophysical techniques
Alix|CD9|MHCII|MHCI|CD86|LAMP2
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 214
MISEV standards
EM
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
EM
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
EM
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 5
MISEV standards
EM|IEM
EV Biophysical techniques
CD86|CD80
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 11145662    
Organism Mus musculus
Experiment description Mast cell-dependent B and T lymphocyte activation is mediated by the secretion of immunologically active exosomes.
Authors Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, David B, Namane A, Mecheri S
Journal name JIMMU
Publication year 2001
Sample Mast cells
Sample name P815
MC9
Bone marrow-derived mast cells
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
ELISA
7
Experiment ID 6
MISEV standards
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 11306949    
Organism Mus musculus
Experiment description Nonspecific B and T cell-stimulatory activity mediated by mast cells is associated with exosomes.
Authors Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, Namane A, David B, Mecheri S
Journal name IAAI
Publication year 2001
Sample Mast cells
Sample name Bone marrow-derived mast cells
Isolation/purification methods -
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 14
MISEV standards
EM
EV Biophysical techniques
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
EV Enriched markers
DNM
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
9
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Itgb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Itgb2 is involved
No pathways found





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