Gene description for Kpna3
Gene name karyopherin (importin) alpha 3
Gene symbol Kpna3
Other names/aliases IPOA4
Species Mus musculus
 Database cross references - Kpna3
ExoCarta ExoCarta_16648
Vesiclepedia VP_16648
Entrez Gene 16648
UniProt O35344  
 Kpna3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Kpna3
Molecular Function
    protein binding GO:0005515 IPI
    nuclear localization sequence binding GO:0008139 IBA
    nuclear import signal receptor activity GO:0061608 IBA
    nuclear import signal receptor activity GO:0061608 IEA
Biological Process
    protein import into nucleus GO:0006606 ISS
    NLS-bearing protein import into nucleus GO:0006607 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 NAS
    cytosol GO:0005829 NAS
    NLS-dependent protein nuclear import complex GO:0042564 ISO
 Experiment description of studies that identified Kpna3 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Kpna3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Foxp3  
Affinity Capture-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Hnrnpc 15381
Co-fractionation Mus musculus
4 Atg16l1  
Affinity Capture-MS Mus musculus
5 Pou5f1  
Affinity Capture-MS Mus musculus
6 Chd8  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
7 Kpna6  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Kpna3 is involved
No pathways found





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