Gene description for Krt15
Gene name keratin 15
Gene symbol Krt15
Other names/aliases AI528832
K15
Krt1-15
Species Mus musculus
 Database cross references - Krt15
ExoCarta ExoCarta_16665
Vesiclepedia VP_16665
Entrez Gene 16665
UniProt Q61414  
 Krt15 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Krt15
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    scaffold protein binding GO:0097110 ISO
Biological Process
    epithelial cell differentiation GO:0030855 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cytoskeleton GO:0005856 IBA
    intermediate filament GO:0005882 IEA
 Experiment description of studies that identified Krt15 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Krt15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Lnx1  
Affinity Capture-MS Mus musculus
3 Nphp4  
Affinity Capture-MS Mus musculus
4 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
5 Krt81 64818
Co-fractionation Mus musculus
6 Ahi1  
Affinity Capture-MS Mus musculus
7 Rhpn1  
Co-fractionation Mus musculus
8 Krt80  
Co-fractionation Mus musculus
9 Krt79  
Co-fractionation Mus musculus
10 Mks1  
Affinity Capture-MS Mus musculus
11 Iqcb1  
Affinity Capture-MS Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
13 Cysrt1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Krt15 is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Formation of the cornified envelope IEA Reactome
Keratinization IEA Reactome





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