Gene description for Krt81
Gene name keratin 81
Gene symbol Krt81
Other names/aliases AA589625
Krt2-19
Species Mus musculus
 Database cross references - Krt81
ExoCarta ExoCarta_64818
Vesiclepedia VP_64818
Entrez Gene 64818
UniProt Q9ERE2  
 Krt81 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Krt81
Molecular Function
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    keratinization GO:0031424 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 IEA
 Experiment description of studies that identified Krt81 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Krt81
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nos2  
Co-fractionation Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
3 Traf3  
Proximity Label-MS Mus musculus
4 Krt15 16665
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Krt81 is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Formation of the cornified envelope IEA Reactome
Keratinization IEA Reactome





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