Gene description for Lgals3
Gene name lectin, galactose binding, soluble 3
Gene symbol Lgals3
Other names/aliases GBP
L-34
Mac-2
gal3
Species Mus musculus
 Database cross references - Lgals3
ExoCarta ExoCarta_16854
Entrez Gene 16854
 Lgals3 identified in exosomes derived from the following tissue/cell type
Dendritic cells 11390481    
Microglia 16081791    
 Gene ontology annotations for Lgals3
Molecular Function
    advanced glycation end-product receptor activity GO:0050785 ISO
    poly(A) RNA binding GO:0044822 ISO
    protein binding GO:0005515 IPI
    Fc-gamma receptor I complex binding GO:0034988 ISO
    laminin binding GO:0043236 ISO
    carbohydrate binding GO:0030246 IEA
    monosaccharide binding GO:0048029 ISO
    IgE binding GO:0019863 ISO
    chemoattractant activity GO:0042056 ISO
    disaccharide binding GO:0048030 ISO
Biological Process
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 ISO
    positive regulation of mononuclear cell migration GO:0071677 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive chemotaxis GO:0050918 ISO
    positive regulation of angiogenesis GO:0045766 ISO
    regulation of T cell proliferation GO:0042129 ISO
    extracellular matrix organization GO:0030198 IGI
    positive regulation of calcium ion import GO:0090280 ISO
    negative regulation of endocytosis GO:0045806 ISO
    immune system process GO:0002376 IEA
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 ISO
    negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:2001189 IMP
    cell differentiation GO:0030154 IEA
    monocyte chemotaxis GO:0002548 ISO
    RNA splicing GO:0008380 IEA
    regulation of T cell apoptotic process GO:0070232 ISO
    positive regulation of cell proliferation GO:0008284 ISO
    neutrophil chemotaxis GO:0030593 ISO
    innate immune response GO:0045087 IEA
    mononuclear cell migration GO:0071674 ISO
    positive regulation of serotonin secretion GO:0014064 ISO
    skeletal system development GO:0001501 IGI
    negative regulation of immunological synapse formation GO:2000521 IMP
    mRNA processing GO:0006397 IEA
    macrophage chemotaxis GO:0048246 ISO
    negative regulation of cell proliferation in bone marrow GO:1903769 ISO
    eosinophil chemotaxis GO:0048245 ISO
    negative regulation of T cell receptor signaling pathway GO:0050860 IMP
    signal transduction GO:0007165 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
    extracellular region GO:0005576 IEA
    cell surface GO:0009986 ISO
    proteinaceous extracellular matrix GO:0005578 IDA
    extracellular space GO:0005615 ISO
    immunological synapse GO:0001772 ISO
    external side of plasma membrane GO:0009897 IDA
    mitochondrial inner membrane GO:0005743 ISO
    extracellular matrix GO:0031012 ISO
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
    spliceosomal complex GO:0005681 IEA
 Experiment description of studies that identified Lgals3 in exosomes
1
Experiment ID 10
ISEV standards
EM
EV Biophysical techniques
Alix
EV Cytosolic markers
CD9|MHCII|MHCI|CD86|LAMP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 14
ISEV standards
EM
EV Biophysical techniques
RAB7|RAB11
EV Cytosolic markers
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
DNM
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Lgals3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lgals3 16854
Two-hybrid Mus musculus
Two-hybrid Mus musculus
2 Ncoa3  
Two-hybrid Mus musculus
3 Sufu  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
4 Pias1  
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Lgals3 is involved
No pathways found





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