Gene description for Anxa1
Gene name annexin A1
Gene symbol Anxa1
Other names/aliases Anx-1
Anx-A1
C430014K04Rik
Lpc-1
Lpc1
Species Mus musculus
 Database cross references - Anxa1
ExoCarta ExoCarta_16952
Vesiclepedia VP_16952
Entrez Gene 16952
UniProt P10107  
 Anxa1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Embryonic fibroblasts 18494037    
Faeces 33431899    
Faeces 33431899    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Anxa1
Molecular Function
    phosphatidylserine binding GO:0001786 IBA
    single-stranded DNA binding GO:0003697 ISO
    single-stranded DNA binding GO:0003697 ISO
    single-stranded RNA binding GO:0003727 ISO
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 ISO
    calcium-dependent phospholipid binding GO:0005544 IBA
    calcium-dependent phospholipid binding GO:0005544 ISO
    calcium-dependent phospholipid binding GO:0005544 ISS
    lipid binding GO:0008289 ISO
    phospholipase A2 inhibitor activity GO:0019834 IEA
    phospholipase A2 inhibitor activity GO:0019834 ISO
    double-stranded DNA helicase activity GO:0036121 ISO
    identical protein binding GO:0042802 ISO
    calcium-dependent protein binding GO:0048306 IEA
    DNA/DNA annealing activity GO:1990814 ISO
Biological Process
    neutrophil homeostasis GO:0001780 IMP
    neutrophil homeostasis GO:0001780 ISO
    adaptive immune response GO:0002250 IEA
    monocyte chemotaxis GO:0002548 ISO
    monocyte chemotaxis GO:0002548 ISS
    regulation of leukocyte migration GO:0002685 IMP
    phagocytosis GO:0006909 IBA
    phagocytosis GO:0006909 IMP
    inflammatory response GO:0006954 IMP
    signal transduction GO:0007165 IBA
    signal transduction GO:0007165 IMP
    cell surface receptor signaling pathway GO:0007166 ISO
    G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger GO:0007187 ISO
    G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger GO:0007187 ISS
    regulation of cell shape GO:0008360 ISO
    regulation of cell shape GO:0008360 ISS
    myoblast migration involved in skeletal muscle regeneration GO:0014839 IDA
    peptide cross-linking GO:0018149 ISO
    actin cytoskeleton organization GO:0030036 ISO
    actin cytoskeleton organization GO:0030036 ISS
    insulin secretion GO:0030073 ISO
    keratinocyte differentiation GO:0030216 IEA
    keratinocyte differentiation GO:0030216 ISO
    positive regulation of vesicle fusion GO:0031340 IEA
    positive regulation of vesicle fusion GO:0031340 ISO
    positive regulation of prostaglandin biosynthetic process GO:0031394 ISO
    DNA duplex unwinding GO:0032508 ISO
    regulation of interleukin-1 production GO:0032652 IMP
    negative regulation of interleukin-8 production GO:0032717 IEA
    negative regulation of interleukin-8 production GO:0032717 ISO
    positive regulation of interleukin-2 production GO:0032743 ISO
    positive regulation of interleukin-2 production GO:0032743 ISS
    positive regulation of neutrophil apoptotic process GO:0033031 IMP
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IEA
    cellular response to vascular endothelial growth factor stimulus GO:0035924 ISO
    positive regulation of T cell proliferation GO:0042102 ISO
    positive regulation of T cell proliferation GO:0042102 ISS
    neutrophil activation GO:0042119 ISO
    neutrophil activation GO:0042119 ISS
    regulation of cell population proliferation GO:0042127 IMP
    positive regulation of apoptotic process GO:0043065 ISO
    innate immune response GO:0045087 IEA
    positive regulation of T-helper 1 cell differentiation GO:0045627 ISO
    positive regulation of T-helper 1 cell differentiation GO:0045627 ISS
    negative regulation of T-helper 2 cell differentiation GO:0045629 ISO
    negative regulation of T-helper 2 cell differentiation GO:0045629 ISS
    negative regulation of exocytosis GO:0045920 ISO
    negative regulation of exocytosis GO:0045920 ISS
    alpha-beta T cell differentiation GO:0046632 IMP
    regulation of hormone secretion GO:0046883 ISO
    regulation of hormone secretion GO:0046883 ISS
    arachidonate secretion GO:0050482 IMP
    negative regulation of protein secretion GO:0050709 ISO
    regulation of inflammatory response GO:0050727 IMP
    cellular response to hydrogen peroxide GO:0070301 ISO
    prolactin secretion GO:0070459 ISO
    cellular response to glucocorticoid stimulus GO:0071385 IBA
    cellular response to glucocorticoid stimulus GO:0071385 IMP
    cellular response to glucocorticoid stimulus GO:0071385 ISO
    granulocyte chemotaxis GO:0071621 ISO
    granulocyte chemotaxis GO:0071621 ISS
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 IEA
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 ISO
    positive regulation of wound healing GO:0090303 ISO
    positive regulation of wound healing GO:0090303 ISS
    neutrophil clearance GO:0097350 IEA
    neutrophil clearance GO:0097350 ISO
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IMP
Subcellular Localization
    cornified envelope GO:0001533 IDA
    cornified envelope GO:0001533 ISO
    phagocytic cup GO:0001891 IEA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    actin filament GO:0005884 IEA
    actin filament GO:0005884 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    vesicle membrane GO:0012506 IBA
    basolateral plasma membrane GO:0016323 IEA
    apical plasma membrane GO:0016324 IDA
    lateral plasma membrane GO:0016328 IDA
    motile cilium GO:0031514 IDA
    early endosome membrane GO:0031901 ISS
    mitochondrial membrane GO:0031966 ISO
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 IDA
    mast cell granule GO:0042629 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 ISO
    extracellular exosome GO:0070062 ISS
    synaptic membrane GO:0097060 ISO
 Experiment description of studies that identified Anxa1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
4
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
5
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
6
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
7
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
11
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Anxa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ywhaq 22630
Co-fractionation Mus musculus
2 S100a10 20194
Co-fractionation Mus musculus
3 Mettl21e  
Affinity Capture-MS Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Nphp4  
Affinity Capture-MS Mus musculus
6 Chuk  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Tfe3  
Affinity Capture-MS Mus musculus
9 Uchl5 56207
Co-fractionation Mus musculus
10 Nphp1  
Affinity Capture-MS Mus musculus
11 Dysf  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 Cd274 60533
Protein-peptide Mus musculus
13 Fancd2  
Affinity Capture-MS Mus musculus
14 Nbr1  
Affinity Capture-Western Mus musculus
15 Tgm2  
Co-fractionation Mus musculus
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