Gene description for Nck1
Gene name non-catalytic region of tyrosine kinase adaptor protein 1
Gene symbol Nck1
Other names/aliases 6330586M15Rik
D230010O13Rik
Nck
Nck-1
Species Mus musculus
 Database cross references - Nck1
ExoCarta ExoCarta_17973
Vesiclepedia VP_17973
Entrez Gene 17973
UniProt Q99M51  
 Nck1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nck1
Molecular Function
    receptor binding GO:0005102 ISO
    protein kinase regulator activity GO:0019887 IEA
    protein binding GO:0005515 IPI
    eukaryotic initiation factor eIF2 binding GO:0071074 ISO
    receptor tyrosine kinase binding GO:0030971 ISO
    protein kinase inhibitor activity GO:0004860 ISO
    protein domain specific binding GO:0019904 IPI
Biological Process
    actin filament organization GO:0007015 IMP
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 ISO
    positive regulation of cap-independent translational initiation GO:1903679 ISO
    negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress GO:1990441 ISO
    cell migration GO:0016477 IMP
    response to other organism GO:0051707 IMP
    negative regulation of protein kinase activity GO:0006469 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    negative regulation of cell death GO:0060548 ISO
    lamellipodium assembly GO:0030032 IMP
    substrate-dependent cell migration, cell extension GO:0006930 IGI
    positive regulation of T cell proliferation GO:0042102 ISO
    negative regulation of PERK-mediated unfolded protein response GO:1903898 ISO
    positive regulation of cap-dependent translational initiation GO:1903676 ISO
    negative regulation of peptidyl-serine phosphorylation GO:0033137 ISO
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 ISO
    T cell activation GO:0042110 ISO
    regulation of translation GO:0006417 IEA
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 ISO
    positive regulation of actin filament polymerization GO:0030838 ISO
    peptidyl-serine dephosphorylation GO:0070262 ISO
Subcellular Localization
    ribosome GO:0005840 ISO
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 IEA
    protein phosphatase type 1 complex GO:0000164 ISO
    vesicle membrane GO:0012506 IDA
    endoplasmic reticulum GO:0005783 ISO
    cell-cell junction GO:0005911 IDA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Nck1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nck1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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