Gene description for Pde8a
Gene name phosphodiesterase 8A
Gene symbol Pde8a
Other names/aliases AI551852
Pde8
Species Mus musculus
 Database cross references - Pde8a
ExoCarta ExoCarta_18584
Vesiclepedia VP_18584
Entrez Gene 18584
UniProt O88502  
 Pde8a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pde8a
Molecular Function
    3',5'-cyclic-AMP phosphodiesterase activity GO:0004115 IBA
    3',5'-cyclic-AMP phosphodiesterase activity GO:0004115 IDA
    3',5'-cyclic-AMP phosphodiesterase activity GO:0004115 ISO
    kinase binding GO:0019900 IEA
    kinase binding GO:0019900 ISO
    protein kinase activator activity GO:0030295 IEA
    protein kinase activator activity GO:0030295 ISO
    metal ion binding GO:0046872 IEA
    3',5'-cyclic-GMP phosphodiesterase activity GO:0047555 IBA
    3',5'-cyclic-GMP phosphodiesterase activity GO:0047555 IEA
    3',5'-cyclic-GMP phosphodiesterase activity GO:0047555 ISO
Biological Process
    cAMP catabolic process GO:0006198 IEA
    signal transduction GO:0007165 IEA
    cAMP-mediated signaling GO:0019933 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 IBA
    cellular response to epidermal growth factor stimulus GO:0071364 IMP
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
 Experiment description of studies that identified Pde8a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pde8a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cers2  
Co-fractionation Mus musculus
2 Slc22a21  
Co-fractionation Mus musculus
3 Smarcd1  
Co-fractionation Mus musculus
4 Cyp2u1  
Co-fractionation Mus musculus
5 Cnga3  
Co-fractionation Mus musculus
6 Slc7a5 20539
Co-fractionation Mus musculus
7 Cyp4b1  
Co-fractionation Mus musculus
8 Pde10a  
Co-fractionation Mus musculus
9 Cyp4f18  
Co-fractionation Mus musculus
10 Cnga1  
Co-fractionation Mus musculus
11 Cyp2c54  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Pde8a is involved
PathwayEvidenceSource
G alpha (s) signalling events IEA Reactome
GPCR downstream signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome





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