Gene description for Slc7a5
Gene name solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Gene symbol Slc7a5
Other names/aliases 4F2LC
D0H16S474E
LAT1
TA1
Species Mus musculus
 Database cross references - Slc7a5
ExoCarta ExoCarta_20539
Vesiclepedia VP_20539
Entrez Gene 20539
UniProt Q9Z127  
 Slc7a5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc7a5
Molecular Function
    protein binding GO:0005515 IPI
    amino acid transmembrane transporter activity GO:0015171 ISO
    aromatic amino acid transmembrane transporter activity GO:0015173 ISO
    neutral L-amino acid transmembrane transporter activity GO:0015175 IBA
    neutral L-amino acid transmembrane transporter activity GO:0015175 ISO
    L-amino acid transmembrane transporter activity GO:0015179 IBA
    L-amino acid transmembrane transporter activity GO:0015179 IDA
    L-leucine transmembrane transporter activity GO:0015190 ISO
    L-leucine transmembrane transporter activity GO:0015190 ISS
    L-tryptophan transmembrane transporter activity GO:0015196 ISO
    L-tryptophan transmembrane transporter activity GO:0015196 ISS
    antiporter activity GO:0015297 ISO
    antiporter activity GO:0015297 ISS
    thyroid hormone transmembrane transporter activity GO:0015349 IEA
    thyroid hormone transmembrane transporter activity GO:0015349 ISO
Biological Process
    amino acid transmembrane transport GO:0003333 IBA
    negative regulation of autophagy GO:0010507 IEA
    negative regulation of autophagy GO:0010507 ISO
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    response to muscle activity GO:0014850 IEA
    neutral amino acid transport GO:0015804 IBA
    neutral amino acid transport GO:0015804 ISO
    L-amino acid transport GO:0015807 IDA
    L-amino acid transport GO:0015807 ISO
    isoleucine transport GO:0015818 IEA
    isoleucine transport GO:0015818 ISO
    L-leucine transport GO:0015820 IDA
    L-leucine transport GO:0015820 ISO
    methionine transport GO:0015821 IEA
    methionine transport GO:0015821 ISO
    phenylalanine transport GO:0015823 IEA
    phenylalanine transport GO:0015823 ISO
    proline transport GO:0015824 IEA
    proline transport GO:0015824 ISO
    tryptophan transport GO:0015827 ISO
    tyrosine transport GO:0015828 IEA
    tyrosine transport GO:0015828 ISO
    valine transport GO:0015829 IEA
    valine transport GO:0015829 ISO
    alanine transport GO:0032328 IEA
    alanine transport GO:0032328 ISO
    positive regulation of type II interferon production GO:0032729 IEA
    positive regulation of type II interferon production GO:0032729 ISO
    positive regulation of interleukin-17 production GO:0032740 IEA
    positive regulation of interleukin-17 production GO:0032740 ISO
    positive regulation of interleukin-4 production GO:0032753 IEA
    positive regulation of interleukin-4 production GO:0032753 ISO
    cellular response to glucose starvation GO:0042149 IEA
    xenobiotic transport GO:0042908 IEA
    xenobiotic transport GO:0042908 ISO
    response to hyperoxia GO:0055093 IEA
    positive regulation of glial cell proliferation GO:0060252 IEA
    positive regulation of glial cell proliferation GO:0060252 ISO
    thyroid hormone transport GO:0070327 IEA
    thyroid hormone transport GO:0070327 ISO
    cellular response to lipopolysaccharide GO:0071222 IEA
    amino acid import across plasma membrane GO:0089718 ISO
    liver regeneration GO:0097421 IEA
    L-histidine transport GO:1902024 IEA
    L-histidine transport GO:1902024 ISO
    cellular response to L-arginine GO:1903577 IEA
    L-leucine import across plasma membrane GO:1903801 IEA
    L-leucine import across plasma membrane GO:1903801 ISO
    L-tryptophan transmembrane transport GO:1904556 ISO
    L-tryptophan transmembrane transport GO:1904556 ISS
    negative regulation of vascular associated smooth muscle cell apoptotic process GO:1905460 IEA
    negative regulation of vascular associated smooth muscle cell apoptotic process GO:1905460 ISO
    positive regulation of L-leucine import across plasma membrane GO:1905534 IEA
    positive regulation of L-leucine import across plasma membrane GO:1905534 ISO
Subcellular Localization
    lysosomal membrane GO:0005765 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    basal plasma membrane GO:0009925 ISO
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    apical plasma membrane GO:0016324 ISS
    microvillus membrane GO:0031528 IEA
    microvillus membrane GO:0031528 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    external side of apical plasma membrane GO:0098591 IEA
    external side of apical plasma membrane GO:0098591 ISO
    amino acid transport complex GO:1990184 ISO
    amino acid transport complex GO:1990184 ISS
 Experiment description of studies that identified Slc7a5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc7a5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pde1a  
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Arhgap10  
Co-fractionation Mus musculus
4 Stag3  
Co-fractionation Mus musculus
5 Arhgef18  
Co-fractionation Mus musculus
6 Slc22a1  
Co-fractionation Mus musculus
7 Rapgef5  
Co-fractionation Mus musculus
8 Cyp2j5  
Co-fractionation Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Cers2  
Co-fractionation Mus musculus
11 Smarcd1  
Co-fractionation Mus musculus
12 Clcn4-2  
Co-fractionation Mus musculus
13 Arhgap30  
Co-fractionation Mus musculus
14 Cyp4b1  
Co-fractionation Mus musculus
15 Cyp2c54  
Co-fractionation Mus musculus
16 Pde8a 18584
Co-fractionation Mus musculus
17 Rapgefl1  
Co-fractionation Mus musculus
18 Tmem173  
Proximity Label-MS Mus musculus
19 Slc25a12  
Co-fractionation Mus musculus
20 Lman1  
Co-fractionation Mus musculus
21 Lims2  
Co-fractionation Mus musculus
22 Dock5  
Co-fractionation Mus musculus
23 Pde10a  
Co-fractionation Mus musculus
24 Cyp2f2  
Co-fractionation Mus musculus
25 Sstr3  
Proximity Label-MS Mus musculus
26 Cyp2u1  
Co-fractionation Mus musculus
27 Arhgap23  
Co-fractionation Mus musculus
28 Keap1  
Co-fractionation Mus musculus
29 Cyp21a1  
Co-fractionation Mus musculus
30 Cyp2c29  
Co-fractionation Mus musculus
31 Cybb 13058
Co-fractionation Mus musculus
32 Fancd2  
Affinity Capture-MS Mus musculus
33 Cyp4f18  
Co-fractionation Mus musculus
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