Gene description for Slc7a5
Gene name solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Gene symbol Slc7a5
Other names/aliases 4F2LC
D0H16S474E
LAT1
TA1
Species Mus musculus
 Database cross references - Slc7a5
ExoCarta ExoCarta_20539
Entrez Gene 20539
UniProt Q9Z127  
 Slc7a5 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
 Gene ontology annotations for Slc7a5
Molecular Function
    L-amino acid transmembrane transporter activity GO:0015179 IDA
    L-phenylalanine transmembrane transporter activity GO:0015192 IBA
    amino acid transmembrane transporter activity GO:0015171 IEA
    antiporter activity GO:0015297 IBA
    L-leucine transmembrane transporter activity GO:0015190 IBA
    L-tryptophan transmembrane transporter activity GO:0015196 IBA
Biological Process
    cation transmembrane transport GO:0098655 IBA
    amino acid transmembrane transport GO:0003333 IBA
    tryptophan transport GO:0015827 IBA
    L-amino acid transport GO:0015807 IDA
    transport GO:0006810 IEA
    phenylalanine transport GO:0015823 IBA
    multicellular organismal development GO:0007275 IEA
    L-alpha-amino acid transmembrane transport GO:1902475 IBA
    amino acid transport GO:0006865 IEA
    cell differentiation GO:0030154 IEA
    leucine transport GO:0015820 IBA
    nervous system development GO:0007399 IEA
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    nucleolus GO:0005730 ISO
    nucleus GO:0005634 ISO
    integral component of plasma membrane GO:0005887 IBA
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Slc7a5 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Slc7a5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Slc7a5 is involved
PathwayEvidenceSource
Amino acid transport across the plasma membrane IEA Reactome
Basigin interactions IEA Reactome





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