Gene description for Prkcsh
Gene name protein kinase C substrate 80K-H
Gene symbol Prkcsh
Other names/aliases 80K-H
PKCSH
Species Mus musculus
 Database cross references - Prkcsh
ExoCarta ExoCarta_19089
Entrez Gene 19089
UniProt O08795  
 Prkcsh identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Pancreatic cells 19351151    
 Gene ontology annotations for Prkcsh
Molecular Function
    RNA binding GO:0003723 IDA
    metal ion binding GO:0046872 IEA
    protein kinase C binding GO:0005080 ISO
    ion channel binding GO:0044325 ISO
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 IEA
    phosphoprotein binding GO:0051219 ISO
Biological Process
    negative regulation of neuron projection development GO:0010977 IMP
    in utero embryonic development GO:0001701 IMP
    renal system development GO:0072001 IGI
    protein heterooligomerization GO:0051291 ISO
    nitrogen compound metabolic process GO:0006807 IGI
    liver development GO:0001889 IGI
    N-glycan processing GO:0006491 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 TAS
    glucosidase II complex GO:0017177 IPI
 Experiment description of studies that identified Prkcsh in exosomes
1
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prkcsh
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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