Gene description for Prkcsh
Gene name protein kinase C substrate 80K-H
Gene symbol Prkcsh
Other names/aliases 80K-H
PKCSH
Species Mus musculus
 Database cross references - Prkcsh
ExoCarta ExoCarta_19089
Vesiclepedia VP_19089
Entrez Gene 19089
UniProt O08795  
 Prkcsh identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prkcsh
Molecular Function
    RNA binding GO:0003723 IDA
    protein kinase C binding GO:0005080 ISO
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    phosphoprotein binding GO:0051219 ISO
Biological Process
    in utero embryonic development GO:0001701 IMP
    liver development GO:0001889 IBA
    liver development GO:0001889 IGI
    N-glycan processing GO:0006491 IBA
    N-glycan processing GO:0006491 ISO
    N-glycan processing GO:0006491 ISS
    N-glycan processing GO:0006491 TAS
    negative regulation of neuron projection development GO:0010977 IMP
    nitrogen cycle metabolic process GO:0071941 IGI
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 TAS
    glucosidase II complex GO:0017177 IBA
    glucosidase II complex GO:0017177 IDA
    glucosidase II complex GO:0017177 IPI
    glucosidase II complex GO:0017177 ISO
    glucosidase II complex GO:0017177 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    intracellular membrane-bounded organelle GO:0043231 ISS
 Experiment description of studies that identified Prkcsh in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prkcsh
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lppr4 229791
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Trim67  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Trim9  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
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