Gene description for Hspa1a
Gene name heat shock protein 1A
Gene symbol Hspa1a
Other names/aliases Hsp70-3
Hsp70.3
Hsp72
hsp68
hsp70A1
Species Mus musculus
 Database cross references - Hspa1a
ExoCarta ExoCarta_193740
Entrez Gene 193740
UniProt Q61696  
 Hspa1a identified in exosomes derived from the following tissue/cell type
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
 Gene ontology annotations for Hspa1a
Molecular Function
    unfolded protein binding GO:0051082 ISO
    ATPase activity, coupled GO:0042623 ISO
    poly(A) RNA binding GO:0044822 ISO
    G-protein coupled receptor binding GO:0001664 ISO
    enzyme binding GO:0019899 ISO
    ATPase activity GO:0016887 ISO
    C3HC4-type RING finger domain binding GO:0055131 ISO
    ATP binding GO:0005524 ISO
    NF-kappaB binding GO:0051059 ISO
    protein binding involved in protein folding GO:0044183 ISO
    protease binding GO:0002020 ISO
    double-stranded RNA binding GO:0003725 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    heat shock protein binding GO:0031072 ISO
    protein N-terminus binding GO:0047485 ISO
Biological Process
    protein folding GO:0006457 ISO
    telomere maintenance GO:0000723 IMP
    response to heat GO:0009408 IMP
    DNA repair GO:0006281 IMP
Subcellular Localization
    nuclear speck GO:0016607 ISO
    apical plasma membrane GO:0016324 ISO
    ubiquitin ligase complex GO:0000151 ISO
    aggresome GO:0016235 ISO
    vesicle GO:0031982 ISO
    ribonucleoprotein complex GO:0030529 ISO
    COP9 signalosome GO:0008180 ISO
    focal adhesion GO:0005925 ISO
    centriole GO:0005814 ISO
    basolateral plasma membrane GO:0016323 ISO
    inclusion body GO:0016234 ISO
    nucleus GO:0005634 ISO
    cytosol GO:0005829 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    cytoplasm GO:0005737 ISO
    protein complex GO:0043234 ISO
    extracellular exosome GO:0070062 ISO
    mitochondrion GO:0005739 ISS
    blood microparticle GO:0072562 ISO
 Experiment description of studies that identified Hspa1a in exosomes
1
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Hspa1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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