ExoCarta: Gene summary

Credits | Contact

Gene description for S100a9
Gene name	S100 calcium binding protein A9 (calgranulin B)
Gene symbol	S100a9
Other names/aliases	60B8Ag AW546964 BEE22 Cagb GAGB L1Ag MRP14 p14
Species	Mus musculus
Database cross references - S100a9
ExoCarta	ExoCarta_20202
Vesiclepedia	VP_20202
Entrez Gene	20202
UniProt	P31725
S100a9 identified in exosomes derived from the following tissue/cell type
Faeces	33431899
Faeces	33431899

Gene ontology annotations for S100a9

Molecular Function
	microtubule binding	GO:0008017	IEA
	antioxidant activity	GO:0016209	IEA
	Toll-like receptor 4 binding	GO:0035662	IEA
	metal ion binding	GO:0046872	IEA
	RAGE receptor binding	GO:0050786	IEA
	calcium ion binding	GO:0005509	IEA
	zinc ion binding	GO:0008270	IEA
	arachidonic acid binding	GO:0050544	IEA
Biological Process
	apoptotic process	GO:0006915	IEA
	regulation of translation	GO:0006417	IMP
	neutrophil chemotaxis	GO:0030593	ISO
	peptidyl-cysteine S-trans-nitrosylation	GO:0035606	ISO
	positive regulation of intrinsic apoptotic signaling pathway	GO:2001244	ISO
	positive regulation of inflammatory response	GO:0050729	ISO
	autophagy	GO:0006914	ISO
	neutrophil aggregation	GO:0070488	ISO
	regulation of integrin biosynthetic process	GO:0045113	IMP
	chemotaxis	GO:0006935	IEA
	peptidyl-cysteine S-nitrosylation	GO:0018119	IMP
	activation of cysteine-type endopeptidase activity involved in apoptotic process	GO:0006919	ISO
	regulation of inflammatory response	GO:0050727	IEA
	innate immune response	GO:0045087	IEA
	leukocyte chemotaxis	GO:0030595	IMP
	inflammatory response	GO:0006954	IEA
	actin cytoskeleton reorganization	GO:0031532	IMP
	positive regulation of peptide secretion	GO:0002793	IMP
	leukocyte migration involved in inflammatory response	GO:0002523	ISO
	immune system process	GO:0002376	IEA
Subcellular Localization
	cytoskeleton	GO:0005856	IEA
	nucleus	GO:0005634	ISO
	cytoplasm	GO:0005737	IEA
	extracellular space	GO:0005615	ISO
	extracellular exosome	GO:0070062	ISO
	extracellular region	GO:0005576	IEA
	membrane	GO:0016020	IEA
	plasma membrane	GO:0005886	IEA

Experiment description of studies that identified S100a9 in exosomes

Experiment ID

303

MISEV standards
✔ EM	EV Biophysical techniques
✔ Cd63\|Cd81\|Cd9	EV Enriched markers
✘	EV Negative markers
✔ NTA	EV Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33431899

Organism

Mus musculus

Experiment description

High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance

Authors

Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG.

Journal name

Nat Commun

Publication year

2021

Sample

Faeces

Sample name

Intestinal epithelial cells - C57BL/6

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
Lipid
miRNA

Methods used in the study

Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC