Gene description for Atp1b1
Gene name ATPase, Na+/K+ transporting, beta 1 polypeptide
Gene symbol Atp1b1
Other names/aliases Atp4b
Atpb
Atpb-1
NKbeta1
Species Mus musculus
 Database cross references - Atp1b1
ExoCarta ExoCarta_11931
Vesiclepedia VP_11931
Entrez Gene 11931
UniProt P14094  
 Atp1b1 identified in exosomes derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Atp1b1
Molecular Function
    ATPase activator activity GO:0001671 IBA
    ATPase activator activity GO:0001671 IMP
    ATPase activator activity GO:0001671 ISO
    P-type sodium:potassium-exchanging transporter activity GO:0005391 IEA
    P-type sodium:potassium-exchanging transporter activity GO:0005391 ISO
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    protein-macromolecule adaptor activity GO:0030674 IEA
    protein-macromolecule adaptor activity GO:0030674 ISO
    protein heterodimerization activity GO:0046982 ISO
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
Biological Process
    response to hypoxia GO:0001666 ISO
    blastocyst development GO:0001824 IMP
    potassium ion transport GO:0006813 ISO
    sodium ion transport GO:0006814 ISO
    intracellular calcium ion homeostasis GO:0006874 IMP
    intracellular sodium ion homeostasis GO:0006883 IBA
    intracellular sodium ion homeostasis GO:0006883 ISO
    cell adhesion GO:0007155 IEA
    establishment or maintenance of transmembrane electrochemical gradient GO:0010248 NAS
    regulation of gene expression GO:0010468 IMP
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 IMP
    metal ion transport GO:0030001 ISO
    intracellular potassium ion homeostasis GO:0030007 IBA
    intracellular potassium ion homeostasis GO:0030007 ISO
    positive regulation of ATP-dependent activity GO:0032781 ISO
    sodium ion transmembrane transport GO:0035725 ISO
    sodium ion export across plasma membrane GO:0036376 IBA
    sodium ion export across plasma membrane GO:0036376 IMP
    sodium ion export across plasma membrane GO:0036376 ISO
    ATP metabolic process GO:0046034 IEA
    ATP metabolic process GO:0046034 ISO
    protein stabilization GO:0050821 IMP
    protein stabilization GO:0050821 ISO
    relaxation of cardiac muscle GO:0055119 IMP
    cardiac muscle contraction GO:0060048 IMP
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    membrane repolarization GO:0086009 IEA
    membrane repolarization GO:0086009 ISO
    monoatomic cation transmembrane transport GO:0098655 ISO
    positive regulation of potassium ion transmembrane transporter activity GO:1901018 ISO
    proton transmembrane transport GO:1902600 NAS
    regulation of calcium ion transmembrane transport GO:1903169 IMP
    positive regulation of sodium ion export across plasma membrane GO:1903278 IEA
    positive regulation of sodium ion export across plasma membrane GO:1903278 ISO
    positive regulation of potassium ion import across plasma membrane GO:1903288 IEA
    positive regulation of potassium ion import across plasma membrane GO:1903288 ISO
    positive regulation of P-type sodium:potassium-exchanging transporter activity GO:1903408 ISO
    potassium ion import across plasma membrane GO:1990573 IBA
    potassium ion import across plasma membrane GO:1990573 IMP
    potassium ion import across plasma membrane GO:1990573 ISO
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    sodium:potassium-exchanging ATPase complex GO:0005890 IBA
    sodium:potassium-exchanging ATPase complex GO:0005890 IMP
    sodium:potassium-exchanging ATPase complex GO:0005890 ISO
    caveola GO:0005901 ISO
    intercalated disc GO:0014704 IMP
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    lateral plasma membrane GO:0016328 IEA
    lateral plasma membrane GO:0016328 ISO
    T-tubule GO:0030315 IEA
    T-tubule GO:0030315 ISO
    organelle membrane GO:0031090 IEA
    organelle membrane GO:0031090 ISO
    sperm flagellum GO:0036126 IEA
    sperm flagellum GO:0036126 ISO
    sarcolemma GO:0042383 IMP
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Atp1b1 in exosomes
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG.
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG.
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Atp1b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Myh6  
Cross-Linking-MS (XL-MS) Mus musculus
2 Usp48  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Prrt2  
Affinity Capture-MS Mus musculus
5 Prnp 19122
Affinity Capture-MS Mus musculus
6 S100a9 20202
Co-fractionation Mus musculus
7 Mapt  
Affinity Capture-MS Mus musculus
8 Agap2  
Affinity Capture-MS Mus musculus
9 Aph1b  
Affinity Capture-MS Mus musculus
10 Aph1a 226548
Affinity Capture-MS Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Htt  
Affinity Capture-MS Mus musculus
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