Gene description for Serpina1a
Gene name serine (or cysteine) peptidase inhibitor, clade A, member 1A
Gene symbol Serpina1a
Other names/aliases Aat-2
Aat2
Dom1
PI1
Spi1-1
Spi1-3
Species Mus musculus
 Database cross references - Serpina1a
ExoCarta ExoCarta_20700
Entrez Gene 20700
UniProt P07758  
 Serpina1a identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
 Gene ontology annotations for Serpina1a
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 ISO
    glycoprotein binding GO:0001948 ISO
    identical protein binding GO:0042802 ISO
    peptidase inhibitor activity GO:0030414 IEA
    endopeptidase inhibitor activity GO:0004866 ISO
    protease binding GO:0002020 ISO
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of peptidase activity GO:0010466 IEA
    response to peptide hormone GO:0043434 IDA
    negative regulation of endopeptidase activity GO:0010951 ISO
    response to cytokine GO:0034097 IDA
    protein N-linked glycosylation GO:0006487 IDA
    acute-phase response GO:0006953 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 ISO
    endoplasmic reticulum GO:0005783 ISO
    extracellular exosome GO:0070062 ISO
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 ISO
 Experiment description of studies that identified Serpina1a in exosomes
1
Experiment ID 26
ISEV standards
EM
EV Biophysical techniques
Alix|GAPDH|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
 Protein-protein interactions for Serpina1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Serpina1a is involved
PathwayEvidenceSource
Platelet degranulation IEA Reactome





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