Gene description for Serpina1b
Gene name serine (or cysteine) preptidase inhibitor, clade A, member 1B
Gene symbol Serpina1b
Other names/aliases D12Ucla2
Dom2
PI2
Spi1-2
Species Mus musculus
 Database cross references - Serpina1b
ExoCarta ExoCarta_20701
Vesiclepedia VP_20701
Entrez Gene 20701
UniProt P22599  
 Serpina1b identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Serpina1b
Molecular Function
    protease binding GO:0002020 ISO
    endopeptidase inhibitor activity GO:0004866 ISO
    serine-type endopeptidase inhibitor activity GO:0004867 IMP
    serine-type endopeptidase inhibitor activity GO:0004867 ISO
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 ISO
Biological Process
    in utero embryonic development GO:0001701 IMP
    protein N-linked glycosylation GO:0006487 IDA
    acute-phase response GO:0006953 IEA
    response to lead ion GO:0010288 ISO
    response to methanol GO:0033986 ISO
    response to cytokine GO:0034097 IDA
    response to peptide hormone GO:0043434 IDA
    response to chromate GO:0046687 ISO
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Serpina1b in sEVs
1
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Serpina1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Timm44  
Co-fractionation Mus musculus
2 Gpx1  
Co-fractionation Mus musculus
3 Alb 11657
Co-fractionation Mus musculus
4 Ttr  
Co-fractionation Mus musculus
5 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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