Gene description for Phldb2
Gene name pleckstrin homology-like domain, family B, member 2
Gene symbol Phldb2
Other names/aliases AV253284
C820004H04Rik
LL5b
LL5beta
Species Mus musculus
 Database cross references - Phldb2
ExoCarta ExoCarta_208177
Vesiclepedia VP_208177
Entrez Gene 208177
UniProt Q8K1N2  
 Phldb2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Phldb2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    microtubule cytoskeleton organization GO:0000226 IEA
    microtubule cytoskeleton organization GO:0000226 ISO
    regulation of gastrulation GO:0010470 IEA
    regulation of gastrulation GO:0010470 ISO
    regulation of epithelial to mesenchymal transition GO:0010717 IEA
    regulation of epithelial to mesenchymal transition GO:0010717 ISO
    cell migration GO:0016477 IGI
    establishment of cell polarity GO:0030010 IGI
    establishment of protein localization GO:0045184 IEA
    establishment of protein localization GO:0045184 ISO
    negative regulation of stress fiber assembly GO:0051497 IEA
    negative regulation of stress fiber assembly GO:0051497 ISO
    negative regulation of focal adhesion assembly GO:0051895 IEA
    negative regulation of focal adhesion assembly GO:0051895 ISO
    regulation of microtubule cytoskeleton organization GO:0070507 IBA
    regulation of microtubule cytoskeleton organization GO:0070507 IEA
    regulation of microtubule cytoskeleton organization GO:0070507 ISO
    negative regulation of wound healing, spreading of epidermal cells GO:1903690 IEA
    negative regulation of wound healing, spreading of epidermal cells GO:1903690 ISO
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 IEA
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 ISO
Subcellular Localization
    podosome GO:0002102 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    cell leading edge GO:0031252 IEA
    cell leading edge GO:0031252 ISO
    cell projection GO:0042995 IEA
    intermediate filament cytoskeleton GO:0045111 IEA
    intermediate filament cytoskeleton GO:0045111 ISO
    basal cortex GO:0045180 IBA
    basal cortex GO:0045180 IEA
    basal cortex GO:0045180 ISO
 Experiment description of studies that identified Phldb2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Phldb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Flnc 68794
Co-fractionation Mus musculus
2 Zbtb7b  
Affinity Capture-MS Mus musculus
3 Pde4dip  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Phldb2 is involved
No pathways found





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