Gene description for Stam
Gene name signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Gene symbol Stam
Other names/aliases STAM1
Species Mus musculus
 Database cross references - Stam
ExoCarta ExoCarta_20844
Vesiclepedia VP_20844
Entrez Gene 20844
UniProt P70297  
 Stam identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stam
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol binding GO:0035091 IEA
    ubiquitin binding GO:0043130 IEA
    ubiquitin-like protein ligase binding GO:0044389 IEA
    ubiquitin-like protein ligase binding GO:0044389 ISO
Biological Process
    protein transport GO:0015031 IEA
    protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043328 IBA
    positive regulation of exosomal secretion GO:1903543 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
    regulation of extracellular exosome assembly GO:1903551 IEA
    regulation of extracellular exosome assembly GO:1903551 ISO
Subcellular Localization
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    early endosome membrane GO:0031901 IEA
    ESCRT-0 complex GO:0033565 IBA
    ESCRT-0 complex GO:0033565 IEA
    ESCRT-0 complex GO:0033565 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Stam in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stam
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
2 Ube4b  
Co-fractionation Mus musculus
3 Stam2  
Co-fractionation Mus musculus
4 UBC 7316
Reconstituted Complex Homo sapiens
5 Sec31a  
Co-fractionation Mus musculus
6 Usp8  
Co-fractionation Mus musculus
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