Gene description for Stx4a
Gene name syntaxin 4A (placental)
Gene symbol Stx4a
Other names/aliases Stx4
Syn-4
Syn4
Species Mus musculus
 Database cross references - Stx4a
ExoCarta ExoCarta_20909
Vesiclepedia VP_20909
Entrez Gene 20909
UniProt P70452  
 Stx4a identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stx4a
Molecular Function
    Rab GTPase binding GO:0017137 ISO
    SNAP receptor activity GO:0005484 ISO
    spectrin binding GO:0030507 ISO
    sphingomyelin phosphodiesterase activator activity GO:0016230 ISO
    protein binding GO:0005515 IPI
    SNARE binding GO:0000149 ISO
Biological Process
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 ISO
    positive regulation of cell proliferation GO:0008284 ISO
    positive regulation of eosinophil degranulation GO:0043311 ISO
    organelle fusion GO:0048284 ISO
    regulation of exocytosis GO:0017157 ISO
    vesicle-mediated transport GO:0016192 IEA
    positive regulation of immunoglobulin secretion GO:0051024 ISO
    positive regulation of catalytic activity GO:0043085 ISO
    positive regulation of cell migration GO:0030335 ISO
    long-term synaptic potentiation GO:0060291 ISO
    intracellular protein transport GO:0006886 IBA
    positive regulation of cell adhesion GO:0045785 ISO
    synaptic vesicle exocytosis GO:0016079 TAS
    membrane fusion GO:0061025 ISO
    positive regulation of protein localization to cell surface GO:2000010 ISO
    neurotransmitter transport GO:0006836 IEA
    synaptic vesicle fusion to presynaptic membrane GO:0031629 IBA
    protein complex assembly GO:0006461 ISO
    transport GO:0006810 IEA
    vesicle docking GO:0048278 IBA
    synaptic vesicle docking GO:0016081 ISO
    response to hydroperoxide GO:0033194 ISO
    SNARE complex assembly GO:0035493 IDA
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    positive regulation of chemotaxis GO:0050921 ISO
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 ISO
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 ISO
Subcellular Localization
    plasma membrane GO:0005886 ISO
    synapse GO:0045202 ISO
    apical plasma membrane GO:0016324 ISO
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
    intracellular GO:0005622 ISO
    SNARE complex GO:0031201 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    myelin sheath adaxonal region GO:0035749 IDA
    storage vacuole GO:0000322 IDA
    cytoplasm GO:0005737 IDA
    trans-Golgi network GO:0005802 IDA
    lateral loop GO:0043219 IDA
    cell GO:0005623 TAS
    sarcolemma GO:0042383 ISO
    integral component of membrane GO:0016021 IBA
    basolateral plasma membrane GO:0016323 ISO
    cell surface GO:0009986 ISO
    specific granule GO:0042581 ISO
    somatodendritic compartment GO:0036477 ISO
    endomembrane system GO:0012505 IBA
    extracellular space GO:0005615 ISO
    dendritic spine GO:0043197 ISO
    synaptic vesicle GO:0008021 IBA
    endosome GO:0005768 ISO
    lamellipodium GO:0030027 ISO
 Experiment description of studies that identified Stx4a in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stx4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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