Gene description for Cdc42bpb
Gene name CDC42 binding protein kinase beta
Gene symbol Cdc42bpb
Other names/aliases MRCKb
Species Mus musculus
 Database cross references - Cdc42bpb
ExoCarta ExoCarta_217866
Vesiclepedia VP_217866
Entrez Gene 217866
UniProt Q7TT50  
 Cdc42bpb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cdc42bpb
Molecular Function
    magnesium ion binding GO:0000287 ISS
    protein kinase activity GO:0004672 ISO
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISS
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    actin cytoskeleton organization GO:0030036 ISO
    actin cytoskeleton organization GO:0030036 ISS
    actomyosin structure organization GO:0031032 IBA
    actomyosin structure organization GO:0031032 IEA
    actomyosin structure organization GO:0031032 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IEA
    cell-cell junction GO:0005911 ISS
    lamellipodium GO:0030027 ISS
    cell leading edge GO:0031252 ISS
    actomyosin GO:0042641 IBA
    actomyosin GO:0042641 IEA
    actomyosin GO:0042641 ISO
 Experiment description of studies that identified Cdc42bpb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cdc42bpb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Atg5  
Affinity Capture-MS Mus musculus
4 Shank3  
Affinity Capture-MS Mus musculus
5 CPNE1 8904
Reconstituted Complex Homo sapiens
6 CPNE4 131034
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
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