Gene description for Tubb3
Gene name tubulin, beta 3 class III
Gene symbol Tubb3
Other names/aliases 3200002H15Rik
M(beta)3
M(beta)6
Species Mus musculus
 Database cross references - Tubb3
ExoCarta ExoCarta_22152
Entrez Gene 22152
UniProt Q9ERD7  
 Tubb3 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Mov neuroglial cells 15210972    
 Gene ontology annotations for Tubb3
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IEA
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    nucleotide binding GO:0000166 IEA
    peptide binding GO:0042277 ISO
Biological Process
    protein polymerization GO:0051258 IEA
    neuron differentiation GO:0030182 ISO
    microtubule-based process GO:0007017 IEA
    axon guidance GO:0007411 ISO
    mitotic nuclear division GO:0007067 ISO
Subcellular Localization
    protein complex GO:0043234 IEA
    axon GO:0030424 ISO
    neuronal cell body GO:0043025 IDA
    extracellular exosome GO:0070062 ISO
    microtubule cytoskeleton GO:0015630 IDA
    cytoplasm GO:0005737 IDA
    intracellular GO:0005622 IDA
    microtubule GO:0005874 ISO
    cell periphery GO:0071944 IDA
    cytoskeleton GO:0005856 IEA
    nucleus GO:0005634 ISO
    dendrite GO:0030425 IDA
 Experiment description of studies that identified Tubb3 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Tubb3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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