Gene description for Ube2m
Gene name ubiquitin-conjugating enzyme E2M
Gene symbol Ube2m
Other names/aliases 2510040H03Rik
UBC12
Ubc-rs2
Species Mus musculus
 Database cross references - Ube2m
ExoCarta ExoCarta_22192
Vesiclepedia VP_22192
Entrez Gene 22192
UniProt P61082  
 Ube2m identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ube2m
Molecular Function
    protein-glutamic acid ligase activity GO:0070739 IEA
    protein-glycine ligase activity GO:0070735 IEA
    ATP binding GO:0005524 IEA
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    ribosomal S6-glutamic acid ligase activity GO:0018169 ISO
    nucleotide binding GO:0000166 IEA
    ubiquitin-protein transferase activity GO:0004842 ISO
    tubulin-glycine ligase activity GO:0070738 IEA
    NEDD8 transferase activity GO:0019788 ISO
    ligase activity GO:0016874 IEA
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    protein-glycine ligase activity, elongating GO:0070737 IEA
    protein-glycine ligase activity, initiating GO:0070736 IEA
Biological Process
    positive regulation of neuron apoptotic process GO:0043525 ISO
    protein ubiquitination GO:0016567 ISO
    cellular protein modification process GO:0006464 ISO
    protein neddylation GO:0045116 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified Ube2m in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ube2m
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CPNE1 8904
Reconstituted Complex Homo sapiens
2 CPNE4 131034
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which Ube2m is involved
PathwayEvidenceSource
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
TGF-beta receptor signaling activates SMADs IEA Reactome





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