Gene description for NFASC
Gene name neurofascin
Gene symbol NFASC
Other names/aliases NF
NRCAML
Species Homo sapiens
 Database cross references - NFASC
ExoCarta ExoCarta_23114
Vesiclepedia VP_23114
Entrez Gene 23114
HGNC 29866
MIM 609145
UniProt O94856  
 NFASC identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NFASC
Molecular Function
    protein binding GO:0005515 IPI
    cell-cell adhesion mediator activity GO:0098632 IBA
Biological Process
    axon guidance GO:0007411 IBA
    brain development GO:0007420 IBA
    peripheral nervous system development GO:0007422 ISS
    myelination GO:0042552 ISS
    cell-cell adhesion GO:0098609 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    axon GO:0030424 IBA
    paranodal junction GO:0033010 IEA
    node of Ranvier GO:0033268 ISS
    node of Ranvier GO:0033268 TAS
    axon initial segment GO:0043194 ISS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified NFASC in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NFASC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ABI2 10152
Two-hybrid Homo sapiens
2 ABI1 10006
Two-hybrid Homo sapiens
3 DDX58 23586
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml



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