Gene description for Hspb1
Gene name heat shock protein B1
Gene symbol Hspb1
Other names/aliases Hsp25
Hsp27
Species Rattus norvegicus
 Database cross references - Hspb1
ExoCarta ExoCarta_24471
Entrez Gene 24471
 Hspb1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Hspb1
Molecular Function
    identical protein binding GO:0042802 ISO
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 ISO
    poly(A) RNA binding GO:0044822 ISO
    protein kinase C inhibitor activity GO:0008426 ISO
    protein kinase C binding GO:0005080 ISO
    ubiquitin binding GO:0043130 IDA
Biological Process
    platelet aggregation GO:0070527 ISO
    positive regulation of tumor necrosis factor biosynthetic process GO:0042535 ISO
    negative regulation of apoptotic process GO:0043066 TAS
    positive regulation of endothelial cell chemotaxis GO:2001028 ISO
    negative regulation of protein serine/threonine kinase activity GO:0071901 ISO
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISO
    negative regulation of apoptotic signaling pathway GO:2001234 ISO
    regulation of I-kappaB kinase/NF-kappaB signaling GO:0043122 ISO
    positive regulation of interleukin-1 beta production GO:0032731 ISO
    positive regulation of angiogenesis GO:0045766 ISO
    intracellular signal transduction GO:0035556 ISO
    negative regulation of protein kinase activity GO:0006469 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    cellular response to vascular endothelial growth factor stimulus GO:0035924 ISO
    response to virus GO:0009615 ISO
    positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway GO:0038033 ISO
    retina homeostasis GO:0001895 ISO
Subcellular Localization
    focal adhesion GO:0005925 ISO
    plasma membrane GO:0005886 ISO
    contractile fiber GO:0043292 ISO
    extracellular exosome GO:0070062 ISO
    proteasome complex GO:0000502 IDA
    extracellular space GO:0005615 ISO
    Z disc GO:0030018 ISO
    intracellular GO:0005622 ISO
    spindle GO:0005819 IEA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified Hspb1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hspb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hspb1 is involved
No pathways found





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