Gene description for Hspb1
Gene name heat shock protein B1
Gene symbol Hspb1
Other names/aliases Hsp25
Hsp27
Species Rattus norvegicus
 Database cross references - Hspb1
ExoCarta ExoCarta_24471
Vesiclepedia VP_24471
Entrez Gene 24471
 Hspb1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Hspb1
Molecular Function
    protein kinase C binding GO:0005080 IEA
    protein kinase C binding GO:0005080 ISO
    protein binding GO:0005515 IPI
    protein kinase C inhibitor activity GO:0008426 IEA
    protein kinase C inhibitor activity GO:0008426 ISO
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    ubiquitin binding GO:0043130 IDA
    protein folding chaperone GO:0044183 IEA
    protein folding chaperone GO:0044183 ISO
    protein folding chaperone GO:0044183 ISS
    unfolded protein binding GO:0051082 IBA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
Biological Process
    regulation of protein phosphorylation GO:0001932 ISO
    regulation of protein phosphorylation GO:0001932 ISS
    response to ischemia GO:0002931 IEP
    negative regulation of protein kinase activity GO:0006469 ISO
    female pregnancy GO:0007565 IEP
    response to heat GO:0009408 IBA
    response to virus GO:0009615 IEA
    response to virus GO:0009615 ISO
    positive regulation of neuron projection development GO:0010976 IGI
    positive regulation of interleukin-1 beta production GO:0032731 IEA
    positive regulation of interleukin-1 beta production GO:0032731 ISO
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    intracellular signal transduction GO:0035556 IEA
    intracellular signal transduction GO:0035556 ISO
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IEA
    cellular response to vascular endothelial growth factor stimulus GO:0035924 ISO
    response to muscle stretch GO:0035994 IEP
    protein refolding GO:0042026 IBA
    negative regulation of apoptotic process GO:0043066 IBA
    negative regulation of apoptotic process GO:0043066 TAS
    regulation of canonical NF-kappaB signal transduction GO:0043122 IEA
    regulation of canonical NF-kappaB signal transduction GO:0043122 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 IEA
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    negative regulation of regulatory T cell differentiation GO:0045590 IMP
    positive regulation of angiogenesis GO:0045766 IEA
    positive regulation of angiogenesis GO:0045766 ISO
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 ISO
    chaperone-mediated protein folding GO:0061077 IEA
    chaperone-mediated protein folding GO:0061077 ISO
    chaperone-mediated protein folding GO:0061077 ISS
    cellular response to hydrogen peroxide GO:0070301 IEP
    platelet aggregation GO:0070527 ISO
    cellular response to interleukin-11 GO:0071348 IEP
    anterograde axonal protein transport GO:0099641 IEA
    anterograde axonal protein transport GO:0099641 ISO
    anterograde axonal protein transport GO:0099641 ISS
    negative regulation of cellular response to oxidative stress GO:1900408 IMP
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 IEA
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 ISO
    cellular response to butyrate GO:1903545 IEP
    response to angiotensin GO:1990776 IEP
    positive regulation of endothelial cell chemotaxis GO:2001028 IEA
    positive regulation of endothelial cell chemotaxis GO:2001028 ISO
    negative regulation of apoptotic signaling pathway GO:2001234 ISO
Subcellular Localization
    proteasome complex GO:0000502 IDA
    cornified envelope GO:0001533 IEA
    cornified envelope GO:0001533 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    spindle GO:0005819 IEA
    plasma membrane GO:0005886 ISO
    Z disc GO:0030018 IEA
    Z disc GO:0030018 ISO
    axon GO:0030424 IDA
    dendrite GO:0030425 IDA
    M band GO:0031430 IDA
    I band GO:0031674 IDA
    perikaryon GO:0043204 IDA
    contractile muscle fiber GO:0043292 ISO
    cardiac myofibril GO:0097512 IDA
    postsynaptic density membrane GO:0098839 IDA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Hspb1 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hspb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cryab  
Two-hybrid Rattus norvegicus
2 Mapkapk5  
Affinity Capture-Western Rattus norvegicus
3 Hspbap1  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Two-hybrid Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
4 Hspb1 24471
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
5 Ubc  
Two-hybrid Rattus norvegicus
View the network image/svg+xml
 Pathways in which Hspb1 is involved
No pathways found





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