Gene description for Pld2
Gene name phospholipase D2
Gene symbol Pld2
Other names/aliases Pldc
Species Rattus norvegicus
 Database cross references - Pld2
ExoCarta ExoCarta_25097
Vesiclepedia VP_25097
Entrez Gene 25097
UniProt P70498  
 Pld2 identified in sEVs derived from the following tissue/cell type
Basophilic leukemia cells 15304316    
 Gene ontology annotations for Pld2
Molecular Function
    phospholipase D activity GO:0004630 IBA
    phospholipase D activity GO:0004630 IDA
    phospholipase D activity GO:0004630 IEA
    phospholipase D activity GO:0004630 ISO
    protein kinase C binding GO:0005080 IPI
    protein binding GO:0005515 IPI
    phosphatidylinositol binding GO:0035091 IEA
Biological Process
    response to hypoxia GO:0001666 IDA
    G protein-coupled receptor internalization GO:0002031 ISO
    phosphatidic acid biosynthetic process GO:0006654 IEA
    receptor-mediated endocytosis GO:0006898 ISO
    phospholipid catabolic process GO:0009395 IBA
    phospholipid catabolic process GO:0009395 IMP
    response to organic cyclic compound GO:0014070 IDA
    lipid catabolic process GO:0016042 IEA
    positive regulation of cell migration GO:0030335 IMP
    neuron projection development GO:0031175 IMP
    intracellular signal transduction GO:0035556 IEA
    response to hydrogen peroxide GO:0042542 IMP
    positive regulation of mast cell degranulation GO:0043306 IMP
    response to peptide hormone GO:0043434 IMP
    positive regulation of cell adhesion GO:0045785 IMP
    positive regulation of receptor-mediated endocytosis GO:0048260 IMP
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IMP
    regulation of vesicle-mediated transport GO:0060627 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    caveola GO:0005901 IDA
    endomembrane system GO:0012505 IEA
    lamellipodium GO:0030027 IDA
    cytoplasmic vesicle GO:0031410 IBA
    brush border membrane GO:0031526 ISO
    sarcolemma GO:0042383 IDA
 Experiment description of studies that identified Pld2 in sEVs
1
Experiment ID 134
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 15304316    
Organism Rattus norvegicus
Experiment description PLD2 is enriched on exosomes and its activity is correlated to the release of exosomes
Authors "Laulagnier K, Grand D, Dujardin A, Hamdi S, Vincent-Schneider H, Lankar D, Salles JP, Bonnerot C, Perret B, Record M."
Journal name FL
Publication year 2004
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
FACS
 Protein-protein interactions for Pld2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Actb 81822
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
2 Ptk2b  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
3 Gm2a  
Reconstituted Complex Rattus norvegicus
4 SNCA 6622
Affinity Capture-Western Homo sapiens
5 Src  
Affinity Capture-Western Rattus norvegicus
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