Gene description for Cycs
Gene name cytochrome c, somatic
Gene symbol Cycs
Other names/aliases CYCSA
Species Rattus norvegicus
 Database cross references - Cycs
ExoCarta ExoCarta_25309
Entrez Gene 25309
UniProt P62898  
 Cycs identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Cycs
Molecular Function
    lipid binding GO:0008289 TAS
    electron carrier activity GO:0009055 IDA
    electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity GO:0045155 ISO
    metal ion binding GO:0046872 IEA
    heme binding GO:0020037 ISO
Biological Process
    apoptotic process GO:0006915 ISO
    activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c GO:0008635 TAS
    positive regulation of cellular respiration GO:1901857 IMP
    negative regulation of hydrogen peroxide biosynthetic process GO:0010730 IMP
    apoptotic DNA fragmentation GO:0006309 ISO
    glial cell apoptotic process GO:0034349 IEP
    hydrogen peroxide metabolic process GO:0042743 ISO
    mitochondrial electron transport, cytochrome c to oxygen GO:0006123 IDA
    response to oxidative stress GO:0006979 IDA
    ATP synthesis coupled electron transport GO:0042773 IBA
    mitochondrial electron transport, ubiquinol to cytochrome c GO:0006122 IBA
    response to ischemia GO:0002931 IDA
Subcellular Localization
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrion GO:0005739 ISO
    mitochondrial respiratory chain GO:0005746 IC
    nucleus GO:0005634 ISO
    cytosol GO:0005829 ISO
    mitochondrial intermembrane space GO:0005758 IBA
 Experiment description of studies that identified Cycs in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cycs
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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