Gene description for Cycs
Gene name cytochrome c, somatic
Gene symbol Cycs
Other names/aliases CYCSA
Species Rattus norvegicus
 Database cross references - Cycs
ExoCarta ExoCarta_25309
Vesiclepedia VP_25309
Entrez Gene 25309
UniProt P62898  
 Cycs identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Cycs
Molecular Function
    electron transfer activity GO:0009055 IBA
    electron transfer activity GO:0009055 IEA
    enzyme binding GO:0019899 IDA
    heme binding GO:0020037 IEA
    heme binding GO:0020037 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    response to ischemia GO:0002931 IDA
    mitochondrial electron transport, ubiquinol to cytochrome c GO:0006122 IBA
    mitochondrial electron transport, cytochrome c to oxygen GO:0006123 IBA
    mitochondrial electron transport, cytochrome c to oxygen GO:0006123 IMP
    apoptotic process GO:0006915 IEA
    apoptotic process GO:0006915 ISO
    response to oxidative stress GO:0006979 IEP
    response to gravity GO:0009629 IEP
    negative regulation of hydrogen peroxide biosynthetic process GO:0010730 IMP
    response to auditory stimulus GO:0010996 ISO
    glial cell apoptotic process GO:0034349 IEP
    response to carbon monoxide GO:0034465 IEP
    hydrogen peroxide metabolic process GO:0042743 ISO
    response to copper ion GO:0046688 IEP
    positive regulation of cellular respiration GO:1901857 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial intermembrane space GO:0005758 IBA
    mitochondrial intermembrane space GO:0005758 IEA
    mitochondrial intermembrane space GO:0005758 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    apoptosome GO:0043293 ISO
 Experiment description of studies that identified Cycs in exosomes
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cycs
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rad23b  
Affinity Capture-MS Rattus norvegicus
2 Uba52 64156
Affinity Capture-MS Rattus norvegicus
3 Park7 117287
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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