Experiment description of studies that identified Cav1 in exosomes

1 Experiment ID 225 ISEV standards ✔ EM EV Biophysical techniques ✔ GAPDH EV Cytosolic markers ✔ CD63 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25998041     Organism Rattus norvegicus Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats. Authors Lee JE, Moon PG, Lee IK, Baek MC Journal name Protein J Publication year 2015 Sample Adipocytes Sample name Adipocytes Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 92 ISEV standards ✔ EM EV Biophysical techniques ✔ HSP70|GAPDH EV Cytosolic markers ✔ CD9|CD151 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting RatRef-12 Microarray FACS Mass spectrometry [MALDI TOF]

3 Experiment ID 93 ISEV standards ✔ EM EV Biophysical techniques ✔ HSP70|GAPDH EV Cytosolic markers ✔ CD9|CD151 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS-Tspan8 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting

Protein-protein interactions for Cav1

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Cav1 is involved

No pathways found