Gene description for Npm1
Gene name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Gene symbol Npm1
Other names/aliases B23NP
Species Rattus norvegicus
 Database cross references - Npm1
ExoCarta ExoCarta_25498
Entrez Gene 25498
UniProt P13084  
 Npm1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Npm1
Molecular Function
    RNA binding GO:0003723 ISO
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IPI
    histone binding GO:0042393 ISO
    protein kinase inhibitor activity GO:0004860 ISS
    enzyme binding GO:0019899 IPI
    protein kinase binding GO:0019901 ISO
    protein N-terminus binding GO:0047485 ISO
    NF-kappaB binding GO:0051059 ISS
    ribosomal small subunit binding GO:0043024 ISO
    Tat protein binding GO:0030957 ISO
    rRNA binding GO:0019843 ISO
    DNA binding GO:0003677 IDA
    protein heterodimerization activity GO:0046982 ISO
    protein homodimerization activity GO:0042803 ISO
    transcription coactivator activity GO:0003713 ISO
    poly(A) RNA binding GO:0044822 ISO
    unfolded protein binding GO:0051082 ISS
    ribosomal large subunit binding GO:0043023 ISO
Biological Process
    ribosomal large subunit export from nucleus GO:0000055 ISO
    positive regulation of cell proliferation GO:0008284 ISO
    regulation of centrosome duplication GO:0010824 ISO
    cell aging GO:0007569 ISS
    nucleosome assembly GO:0006334 ISO
    cell volume homeostasis GO:0006884 ISO
    positive regulation of centrosome duplication GO:0010825 ISO
    regulation of cell cycle GO:0051726 ISO
    negative regulation of centrosome duplication GO:0010826 ISO
    regulation of centriole replication GO:0046599 ISS
    DNA repair GO:0006281 ISS
    protein oligomerization GO:0051259 ISO
    rRNA export from nucleus GO:0006407 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    regulation of DNA damage response, signal transduction by p53 class mediator GO:0043516 ISO
    protein localization GO:0008104 ISO
    positive regulation of translation GO:0045727 ISS
    centrosome cycle GO:0007098 ISS
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
    positive regulation of DNA replication GO:0045740 IDA
    protein destabilization GO:0031648 ISO
    positive regulation of cellular biosynthetic process GO:0031328 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    ribosomal small subunit export from nucleus GO:0000056 ISO
    regulation of eIF2 alpha phosphorylation by dsRNA GO:0060735 ISS
    protein homooligomerization GO:0051260 ISO
    ribosomal large subunit biogenesis GO:0042273 ISO
    positive regulation of DNA metabolic process GO:0051054 IDA
    regulation of endoribonuclease activity GO:0060699 ISS
    negative regulation of cell proliferation GO:0008285 ISS
    nucleocytoplasmic transport GO:0006913 ISO
    regulation of endodeoxyribonuclease activity GO:0032071 ISS
    positive regulation of protein kinase activity GO:0045860 ISO
    cell growth GO:0016049 ISO
    ribosomal small subunit biogenesis GO:0042274 ISO
    negative regulation of protein kinase activity by regulation of protein phosphorylation GO:0044387 ISS
    positive regulation of catalytic activity GO:0043085 IDA
    regulation of neuron apoptotic process GO:0043523 IMP
Subcellular Localization
    large ribosomal subunit GO:0015934 ISO
    nucleoplasm GO:0005654 ISO
    spindle pole centrosome GO:0031616 ISO
    centrosome GO:0005813 ISS
    nucleolus GO:0005730 ISO
    focal adhesion GO:0005925 ISO
    cytosol GO:0005829 ISO
    membrane GO:0016020 ISO
    small ribosomal subunit GO:0015935 ISO
    intracellular GO:0005622 ISO
    cell GO:0005623 ISO
    granular component GO:0001652 ISO
    cytoplasm GO:0005737 ISS
    nucleus GO:0005634 ISS
    ribonucleoprotein complex GO:0030529 ISO
    nuclear speck GO:0016607 ISO
 Experiment description of studies that identified Npm1 in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Npm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Npm1 is involved
No pathways found





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