Gene description for Cars
Gene name cysteinyl-tRNA synthetase
Gene symbol Cars
Other names/aliases CA3
Species Mus musculus
 Database cross references - Cars
ExoCarta ExoCarta_27267
Vesiclepedia VP_27267
Entrez Gene 27267
UniProt Q9ER72  
 Cars identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cars
Molecular Function
    tRNA binding GO:0000049 ISO
    tRNA binding GO:0000049 ISS
    cysteine-tRNA ligase activity GO:0004817 IBA
    cysteine-tRNA ligase activity GO:0004817 ISO
    cysteine-tRNA ligase activity GO:0004817 ISS
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    cysteinyl-tRNA aminoacylation GO:0006423 IBA
    cysteinyl-tRNA aminoacylation GO:0006423 ISO
    cysteinyl-tRNA aminoacylation GO:0006423 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Cars in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cars
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Thumpd3  
Co-fractionation Mus musculus
2 Luc7l2 192196
Co-fractionation Mus musculus
3 Atg16l1  
Affinity Capture-MS Mus musculus
4 Xpo7  
Co-fractionation Mus musculus
5 Ppm1g  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Cars is involved
No pathways found





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