Gene description for Vps25
Gene name vacuolar protein sorting 25 (yeast)
Gene symbol Vps25
Other names/aliases 1110020N13Rik
AW107467
D11Wsu68e
Species Mus musculus
 Database cross references - Vps25
ExoCarta ExoCarta_28084
Vesiclepedia VP_28084
Entrez Gene 28084
UniProt Q9CQ80  
 Vps25 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vps25
Molecular Function
    structural molecule activity GO:0005198 IBA
    protein homodimerization activity GO:0042803 IBA
    protein homodimerization activity GO:0042803 ISO
Biological Process
    negative regulation of epidermal growth factor-activated receptor activity GO:0007175 ISO
    protein transport GO:0015031 IEA
    protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043328 IBA
    multivesicular body sorting pathway GO:0071985 IEA
Subcellular Localization
    ESCRT II complex GO:0000814 IBA
    ESCRT II complex GO:0000814 IEA
    ESCRT II complex GO:0000814 ISO
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 ISO
    endosome membrane GO:0010008 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Vps25 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vps25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp3cb  
Co-fractionation Mus musculus
2 VPS36 51028
Affinity Capture-MS Homo sapiens
3 Ubc  
Reconstituted Complex Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 SPAG1 6674
Affinity Capture-MS Homo sapiens
7 Snf8 27681
Co-fractionation Mus musculus
8 Vps36 70160
Co-fractionation Mus musculus
9 SNF8 11267
Affinity Capture-MS Homo sapiens
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