Gene description for Vps36
Gene name vacuolar protein sorting 36 (yeast)
Gene symbol Vps36
Other names/aliases 1700010A24Rik
2210415M20Rik
2810408E15Rik
Eap45
Species Mus musculus
 Database cross references - Vps36
ExoCarta ExoCarta_70160
Vesiclepedia VP_70160
Entrez Gene 70160
UniProt Q91XD6  
 Vps36 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vps36
Molecular Function
    phosphatidylinositol-3-phosphate binding GO:0032266 IEA
    ubiquitin binding GO:0043130 IBA
    ubiquitin binding GO:0043130 IDA
    ubiquitin binding GO:0043130 ISO
Biological Process
    protein transport GO:0015031 IEA
    endosomal transport GO:0016197 IC
    endosome transport via multivesicular body sorting pathway GO:0032509 IEA
    protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043328 IBA
Subcellular Localization
    ESCRT II complex GO:0000814 IBA
    ESCRT II complex GO:0000814 IEA
    ESCRT II complex GO:0000814 ISO
    nucleus GO:0005634 IEA
    lysosome GO:0005764 IDA
    endosome GO:0005768 ISO
    late endosome GO:0005770 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
 Experiment description of studies that identified Vps36 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vps36
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp3cb  
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
Co-crystal Structure Mus musculus
Reconstituted Complex Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 UBC 7316
Reconstituted Complex Homo sapiens
5 Runx2  
Affinity Capture-MS Mus musculus
6 Vps25 28084
Co-fractionation Mus musculus
7 AI314180 230249
Co-fractionation Mus musculus
8 Snf8 27681
Co-fractionation Mus musculus
View the network image/svg+xml



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