Gene description for GPX2
Gene name glutathione peroxidase 2
Gene symbol GPX2
Other names/aliases GI-GPx
GPRP
GPRP-2
GPx-2
GPx-GI
GSHPX-GI
GSHPx-2
Species Homo sapiens
 Database cross references - GPX2
ExoCarta ExoCarta_2877
Entrez Gene 2877
HGNC 4554
MIM 138319
UniProt P18283  
 GPX2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GPX2
Molecular Function
    glutathione peroxidase activity GO:0004602 TAS
    electron carrier activity GO:0009055 TAS
Biological Process
    response to symbiotic bacterium GO:0009609 IEA
    negative regulation of inflammatory response to antigenic stimulus GO:0002862 IEA
    interaction with symbiont GO:0051702 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    oxidation-reduction process GO:0055114 IEA
    response to reactive oxygen species GO:0000302 TAS
    gene expression GO:0010467 TAS
    temperature homeostasis GO:0001659 IEA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GPX2 in exosomes
1
Experiment ID 283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 234
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GPX2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GPX2 2877
Invitro Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml



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