ExoCarta: Gene summary

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Gene description for Thbs4
Gene name	thrombospondin 4
Gene symbol	Thbs4
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Thbs4
ExoCarta	ExoCarta_29220
Entrez Gene	29220
Thbs4 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Thbs4

Molecular Function
	collagen binding	GO:0005518	IDA
	calcium ion binding	GO:0005509	ISO
	fibronectin binding	GO:0001968	IDA
	heparin binding	GO:0008201	ISO
	laminin-1 binding	GO:0043237	IDA
	integrin binding	GO:0005178	ISO
	growth factor activity	GO:0008083	IEA
Biological Process
	positive regulation of endothelial cell proliferation	GO:0001938	ISO
	positive regulation of neutrophil chemotaxis	GO:0090023	ISO
	negative regulation of angiogenesis	GO:0016525	ISO
	response to endoplasmic reticulum stress	GO:0034976	ISS
	protein homooligomerization	GO:0051260	IDA
	positive regulation of cell division	GO:0051781	IEA
	response to unfolded protein	GO:0006986	IEA
	positive regulation of peptidyl-tyrosine phosphorylation	GO:0050731	ISO
	behavioral response to pain	GO:0048266	ISO
	nervous system development	GO:0007399	IDA
	endothelial cell-cell adhesion	GO:0071603	ISO
	regulation of tissue remodeling	GO:0034103	ISS
	neuron projection morphogenesis	GO:0048812	IDA
	cell adhesion	GO:0007155	IEA
	tissue remodeling	GO:0048771	IEA
	myoblast migration	GO:0051451	ISO
Subcellular Localization
	extracellular exosome	GO:0070062	ISO
	neuromuscular junction	GO:0031594	IDA
	proteinaceous extracellular matrix	GO:0005578	IDA
	extracellular space	GO:0005615	ISS
	basement membrane	GO:0005604	ISO
	endoplasmic reticulum	GO:0005783	ISS
	sarcoplasmic reticulum	GO:0016529	ISS
	extracellular matrix	GO:0031012	ISS

Experiment description of studies that identified Thbs4 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting