Gene description for Thbs4
Gene name thrombospondin 4
Gene symbol Thbs4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Thbs4
ExoCarta ExoCarta_29220
Vesiclepedia VP_29220
Entrez Gene 29220
 Thbs4 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Thbs4
Molecular Function
    fibronectin binding GO:0001968 IDA
    integrin binding GO:0005178 IEA
    integrin binding GO:0005178 ISO
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    collagen binding GO:0005518 IDA
    growth factor activity GO:0008083 IEA
    heparin binding GO:0008201 IEA
    heparin binding GO:0008201 ISO
    identical protein binding GO:0042802 IPI
    laminin-1 binding GO:0043237 IDA
Biological Process
    positive regulation of endothelial cell proliferation GO:0001938 IEA
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    response to unfolded protein GO:0006986 IEA
    cell adhesion GO:0007155 IEA
    signal transduction GO:0007165 IEA
    nervous system development GO:0007399 IDA
    negative regulation of angiogenesis GO:0016525 IEA
    negative regulation of angiogenesis GO:0016525 ISO
    regulation of tissue remodeling GO:0034103 IEA
    regulation of tissue remodeling GO:0034103 ISO
    regulation of tissue remodeling GO:0034103 ISS
    response to endoplasmic reticulum stress GO:0034976 IBA
    response to endoplasmic reticulum stress GO:0034976 IEA
    response to endoplasmic reticulum stress GO:0034976 ISO
    response to endoplasmic reticulum stress GO:0034976 ISS
    behavioral response to pain GO:0048266 IEA
    behavioral response to pain GO:0048266 IMP
    behavioral response to pain GO:0048266 ISO
    tissue remodeling GO:0048771 IEA
    neuron projection morphogenesis GO:0048812 IDA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    myoblast migration GO:0051451 IEA
    myoblast migration GO:0051451 ISO
    positive regulation of cell division GO:0051781 IEA
    endothelial cell-cell adhesion GO:0071603 IEA
    endothelial cell-cell adhesion GO:0071603 ISO
    positive regulation of neutrophil chemotaxis GO:0090023 IEA
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
Subcellular Localization
    extracellular region GO:0005576 IEA
    basement membrane GO:0005604 IEA
    basement membrane GO:0005604 ISO
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    sarcoplasmic reticulum GO:0016529 IEA
    sarcoplasmic reticulum GO:0016529 ISO
    sarcoplasmic reticulum GO:0016529 ISS
    neuromuscular junction GO:0031594 IDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 ISO
    collagen-containing extracellular matrix GO:0062023 ISS
 Experiment description of studies that identified Thbs4 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Thbs4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rad23b  
Affinity Capture-MS Rattus norvegicus
2 FN1 280794
Reconstituted Complex Bos taurus
3 Matn2  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Thbs4 is involved
No pathways found





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