Gene description for Actc1
Gene name actin, alpha, cardiac muscle 1
Gene symbol Actc1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Actc1
ExoCarta ExoCarta_29275
Vesiclepedia VP_29275
Entrez Gene 29275
UniProt P68035  
 Actc1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Actc1
Molecular Function
    microfilament motor activity GO:0000146 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    hydrolase activity GO:0016787 IEA
    myosin binding GO:0017022 IBA
    myosin binding GO:0017022 ISO
    protein kinase binding GO:0019901 IEA
Biological Process
    actin filament organization GO:0007015 IBA
    regulation of blood pressure GO:0008217 IEA
    response to xenobiotic stimulus GO:0009410 IEP
    response to virus GO:0009615 IEA
    positive regulation of gene expression GO:0010628 ISS
    vascular associated smooth muscle contraction GO:0014829 IEA
    actin filament-based movement GO:0030048 ISO
    skeletal muscle thin filament assembly GO:0030240 ISO
    actomyosin structure organization GO:0031032 ISO
    actin-myosin filament sliding GO:0033275 IBA
    actin-myosin filament sliding GO:0033275 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    response to ethanol GO:0045471 IEP
    cardiac myofibril assembly GO:0055003 ISO
    cardiac muscle tissue morphogenesis GO:0055008 ISO
    heart contraction GO:0060047 IBA
    heart contraction GO:0060047 ISO
    cardiac muscle contraction GO:0060048 IDA
    actin-mediated cell contraction GO:0070252 ISO
    glomerular mesangial cell development GO:0072144 IEA
    mesenchyme migration GO:0090131 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytoskeleton GO:0005856 IEA
    actin filament GO:0005884 IBA
    actin filament GO:0005884 ISO
    actin cytoskeleton GO:0015629 IBA
    actin cytoskeleton GO:0015629 IEA
    sarcomere GO:0030017 IBA
    sarcomere GO:0030017 ISO
    lamellipodium GO:0030027 ISS
    filopodium GO:0030175 ISS
    smooth muscle contractile fiber GO:0030485 IEA
    motile cilium GO:0031514 IEA
    I band GO:0031674 ISO
    protein-containing complex GO:0032991 IEA
    cell body GO:0044297 ISS
    synapse GO:0045202 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Actc1 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Actc1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snca  
Affinity Capture-MS Rattus norvegicus
2 Rad23b  
Affinity Capture-MS Rattus norvegicus
3 Atxn3  
Affinity Capture-MS Mus musculus
4 Park7 117287
Affinity Capture-MS Rattus norvegicus
5 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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