Gene description for HAS2
Gene name hyaluronan synthase 2
Gene symbol HAS2
Other names/aliases -
Species Homo sapiens
 Database cross references - HAS2
ExoCarta ExoCarta_3037
Vesiclepedia VP_3037
Entrez Gene 3037
HGNC 4819
MIM 601636
UniProt Q92819  
 HAS2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HAS2
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IDA
    hyaluronan synthase activity GO:0050501 IBA
    hyaluronan synthase activity GO:0050501 IDA
    hyaluronan synthase activity GO:0050501 IMP
    hyaluronan synthase activity GO:0050501 ISS
Biological Process
    polysaccharide biosynthetic process GO:0000271 IBA
    polysaccharide biosynthetic process GO:0000271 ISS
    vasculogenesis GO:0001570 ISS
    kidney development GO:0001822 ISS
    positive regulation of cell population proliferation GO:0008284 IMP
    positive regulation of keratinocyte proliferation GO:0010838 IEA
    positive regulation of smooth muscle cell migration GO:0014911 IEA
    hyaluronan biosynthetic process GO:0030213 IBA
    hyaluronan biosynthetic process GO:0030213 IDA
    hyaluronan biosynthetic process GO:0030213 IEA
    hyaluronan biosynthetic process GO:0030213 IMP
    hyaluronan biosynthetic process GO:0030213 ISS
    positive regulation of cell migration GO:0030335 IMP
    positive regulation of urine volume GO:0035810 ISS
    cellular response to platelet-derived growth factor stimulus GO:0036120 IDA
    atrioventricular canal development GO:0036302 ISS
    estrous cycle GO:0044849 IEA
    positive regulation of keratinocyte migration GO:0051549 IEA
    bone morphogenesis GO:0060349 IEA
    renal water absorption GO:0070295 ISS
    cellular response to interleukin-1 GO:0071347 IEP
    cellular response to tumor necrosis factor GO:0071356 IEP
    cellular response to fluid shear stress GO:0071498 IEP
    extracellular matrix assembly GO:0085029 IBA
    extracellular matrix assembly GO:0085029 ISS
    endocardial cushion to mesenchymal transition GO:0090500 ISS
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of hyaluronan biosynthetic process GO:1900127 IEA
    positive regulation of monocyte aggregation GO:1900625 IMP
    regulation of extracellular matrix assembly GO:1901201 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    lysosome GO:0005764 IEA
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cytoplasmic vesicle GO:0031410 IEA
    plasma membrane raft GO:0044853 IEA
    extracellular vesicle GO:1903561 IDA
 Experiment description of studies that identified HAS2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HAS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HAS2 3037
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 HAS3  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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