Gene description for Gpc4
Gene name glypican 4
Gene symbol Gpc4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gpc4
ExoCarta ExoCarta_317322
Vesiclepedia VP_317322
Entrez Gene 317322
 Gpc4 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Gpc4
Biological Process
    biological_process GO:0008150 ND
    regulation of signal transduction GO:0009966 IEA
    Wnt signaling pathway GO:0016055 ISO
    cell migration GO:0016477 IBA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IBA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IDA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IMP
    synaptic membrane adhesion GO:0099560 IBA
    synaptic membrane adhesion GO:0099560 ISO
    regulation of presynapse assembly GO:1905606 IBA
    regulation of presynapse assembly GO:1905606 IDA
    regulation of presynapse assembly GO:1905606 IMP
Subcellular Localization
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 IBA
    presynaptic membrane GO:0042734 IDA
    presynaptic membrane GO:0042734 IMP
    synapse GO:0045202 IBA
    synapse GO:0045202 ISO
    collagen-containing extracellular matrix GO:0062023 IEA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Gpc4 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gpc4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ptprs  
Reconstituted Complex Rattus norvegicus
Co-fractionation Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
Reconstituted Complex Rattus norvegicus
2 Mib1  
Affinity Capture-MS Rattus norvegicus
3 PTPRS 5802
Reconstituted Complex Homo sapiens
4 Lrrtm4  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Gpc4 is involved
No pathways found





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