Gene description for Gpc4
Gene name glypican 4
Gene symbol Gpc4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gpc4
ExoCarta ExoCarta_317322
Entrez Gene 317322
 Gpc4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Gpc4
Molecular Function
    heparan sulfate proteoglycan binding GO:0043395 IEA
    molecular_function GO:0003674 ND
Biological Process
    biological_process GO:0008150 ND
Subcellular Localization
    nucleus GO:0005634 ISO
    cellular_component GO:0005575 ND
    proteinaceous extracellular matrix GO:0005578 IEA
    extracellular exosome GO:0070062 ISO
    anchored component of membrane GO:0031225 IEA
    external side of plasma membrane GO:0009897 ISO
 Experiment description of studies that identified Gpc4 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gpc4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gpc4 is involved
No pathways found





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