Gene description for RpL5
Gene name Ribosomal protein L5
Gene symbol RpL5
Other names/aliases CG17489
DmelCG17489
E-2d
L5
M(2)40B
Rp L5
Rpl5
dRPL5
yip6
Species Drosophila melanogaster
 Database cross references - RpL5
ExoCarta ExoCarta_3355124
Vesiclepedia VP_3355124
Entrez Gene 3355124
UniProt Q9W5R8  
 RpL5 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for RpL5
Molecular Function
    structural constituent of ribosome GO:0003735 HDA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 NAS
    structural constituent of ribosome GO:0003735 TAS
    protein binding GO:0005515 IPI
    5S rRNA binding GO:0008097 NAS
Biological Process
    cytoplasmic translation GO:0002181 IC
    cytoplasmic translation GO:0002181 TAS
Subcellular Localization
    cytosolic large ribosomal subunit GO:0022625 NAS
    cytosolic large ribosomal subunit GO:0022625 TAS
    cytosolic ribosome GO:0022626 HDA
    cytosolic ribosome GO:0022626 IDA
 Experiment description of studies that identified RpL5 in exosomes
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RpL5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 eIF4E-5  
Two-hybrid Drosophila melanogaster
2 Neto  
Two-hybrid Drosophila melanogaster
3 slgA  
Two-hybrid Drosophila melanogaster
4 Rcd-1r  
Two-hybrid Drosophila melanogaster
5 RASSF8  
Two-hybrid Drosophila melanogaster
6 ebo  
Two-hybrid Drosophila melanogaster
7 squ  
Two-hybrid Drosophila melanogaster
8 kermit  
Two-hybrid Drosophila melanogaster
9 noi  
Two-hybrid Drosophila melanogaster
10 rb  
Two-hybrid Drosophila melanogaster
11 CG2678  
Two-hybrid Drosophila melanogaster
12 CG4520  
Two-hybrid Drosophila melanogaster
13 CG13287  
Two-hybrid Drosophila melanogaster
14 OS9  
Two-hybrid Drosophila melanogaster
15 CycG  
Two-hybrid Drosophila melanogaster
16 fd59A  
Two-hybrid Drosophila melanogaster
17 Fmr1  
Affinity Capture-MS Drosophila melanogaster
Affinity Capture-Western Drosophila melanogaster
Reconstituted Complex Drosophila melanogaster
18 Rbsn-5  
Two-hybrid Drosophila melanogaster
19 M1BP  
Two-hybrid Drosophila melanogaster
20 me31B  
Proximity Label-MS Drosophila melanogaster
21 CG14868  
Two-hybrid Drosophila melanogaster
22 CG5645  
Two-hybrid Drosophila melanogaster
23 CG10032  
Two-hybrid Drosophila melanogaster
24 4E-T  
Two-hybrid Drosophila melanogaster
25 CG14476  
Two-hybrid Drosophila melanogaster
26 ird5  
Affinity Capture-MS Drosophila melanogaster
27 CTPsyn  
Proximity Label-MS Drosophila melanogaster
28 VhaAC39-1  
Two-hybrid Drosophila melanogaster
29 NaCP60E  
Two-hybrid Drosophila melanogaster
30 CG5001  
Two-hybrid Drosophila melanogaster
31 Tab2  
Affinity Capture-MS Drosophila melanogaster
32 EF2 35422
Two-hybrid Drosophila melanogaster
33 Bet3  
Two-hybrid Drosophila melanogaster
34 CG11586  
Two-hybrid Drosophila melanogaster
35 veil  
Two-hybrid Drosophila melanogaster
36 Lk6  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml



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