ExoCarta: Gene summary

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Gene description for RpL5
Gene name	Ribosomal protein L5
Gene symbol	RpL5
Other names/aliases	CG17489 DmelCG17489 E-2d L5 M(2)40B Rp L5 Rpl5 dRPL5 yip6
Species	Drosophila melanogaster
Database cross references - RpL5
ExoCarta	ExoCarta_3355124
Entrez Gene	3355124
UniProt	Q9W5R8
RpL5 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells	23035643

Gene ontology annotations for RpL5

Molecular Function
	RNA binding	GO:0003723	ISS
	protein binding	GO:0005515	IPI
	structural constituent of ribosome	GO:0003735	NAS
	5S rRNA binding	GO:0008097	NAS
Biological Process
	translation	GO:0006412	ISS
	ribosomal large subunit assembly	GO:0000027	ISS
Subcellular Localization
	cytosolic large ribosomal subunit	GO:0022625	NAS
	ribosome	GO:0005840	IDA

Experiment description of studies that identified RpL5 in exosomes

Experiment ID

202

ISEV standards
✔ IEM	EV Biophysical techniques
✔ TSG10\|RAB35	EV Cytosolic markers
✔ FLOT2	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23035643

Organism

Drosophila melanogaster

Experiment description

Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.

Authors

Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.

Journal name

Traffic

Publication year

2012

Sample

Drosophila S2 cells

Sample name

Normal-Drosophila S2 tub-Wg

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for RpL5

	Protein Interactor	ExoCarta ID	Identification method	PubMed	Species
No interactions are found.

Pathways in which RpL5 is involved

Pathway	Evidence	Source
Eukaryotic Translation Termination	IEA	Reactome
Formation of a pool of free 40S subunits	IEA	Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit	IEA	Reactome
L13a-mediated translational silencing of Ceruloplasmin expression	IEA	Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)	IEA	Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)	IEA	Reactome
Peptide chain elongation	IEA	Reactome
SRP-dependent cotranslational protein targeting to membrane	IEA	Reactome

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