Gene description for EF2
Gene name Elongation factor 2
Gene symbol EF2
Other names/aliases CG2238
DmelCG2238
EF-2
EF-2b
EF2b
Ef2
Ef2b
anon-EST:Liang-1.44
chr2L:21668915..21669164
clone 1.44
eEF2
eF2
ef2b
Species Drosophila melanogaster
 Database cross references - EF2
ExoCarta ExoCarta_35422
Entrez Gene 35422
UniProt P13060  
 EF2 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for EF2
Molecular Function
    translation elongation factor activity GO:0003746 NAS
    GTPase activity GO:0003924 IEA
    GTP binding GO:0005525 IEA
Biological Process
    translational elongation GO:0006414 ISS
    mitotic spindle organization GO:0007052 IMP
    mitotic spindle elongation GO:0000022 IMP
    translation GO:0006412 NAS
Subcellular Localization
    lipid particle GO:0005811 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 ISS
    microtubule associated complex GO:0005875 IDA
 Experiment description of studies that identified EF2 in exosomes
1
Experiment ID 202
ISEV standards
IEM
EV Biophysical techniques
TSG10|RAB35
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which EF2 is involved
PathwayEvidenceSource
Peptide chain elongation IEA Reactome
Synthesis of diphthamide-EEF2 IEA Reactome





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