Gene description for EF2
Gene name Elongation factor 2
Gene symbol EF2
Other names/aliases CG2238
DmelCG2238
EF-2
EF-2b
EF2b
Ef2
Ef2b
anon-EST:Liang-1.44
chr2L:21668915..21669164
clone 1.44
eEF2
eF2
ef2b
Species Drosophila melanogaster
 Database cross references - EF2
ExoCarta ExoCarta_35422
Vesiclepedia VP_35422
Entrez Gene 35422
UniProt P13060  
 EF2 identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for EF2
Molecular Function
    translation elongation factor activity GO:0003746 ISS
Biological Process
    translational elongation GO:0006414 ISS
Subcellular Localization
    cytosol GO:0005829 ISS
 Experiment description of studies that identified EF2 in sEVs
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sxl  
Two-hybrid Drosophila melanogaster
2 Inos 35671
Affinity Capture-MS Drosophila melanogaster
3 awd  
Affinity Capture-MS Drosophila melanogaster
4 Iap2  
Affinity Capture-MS Drosophila melanogaster
5 CG10638  
Affinity Capture-MS Drosophila melanogaster
6 tsg  
Two-hybrid Drosophila melanogaster
7 Sec24AB  
Two-hybrid Drosophila melanogaster
8 srpk79D  
Two-hybrid Drosophila melanogaster
9 Sh3beta 38820
Affinity Capture-MS Drosophila melanogaster
10 NimC1  
Two-hybrid Drosophila melanogaster
11 CG8786  
Two-hybrid Drosophila melanogaster
12 loqs  
Affinity Capture-MS Drosophila melanogaster
13 CG8316  
Two-hybrid Drosophila melanogaster
14 Ubx  
Two-hybrid Drosophila melanogaster
Reconstituted Complex Drosophila melanogaster
15 Chd64  
Affinity Capture-MS Drosophila melanogaster
16 mmd  
Two-hybrid Drosophila melanogaster
17 Eno  
Affinity Capture-MS Drosophila melanogaster
18 CG9170  
Two-hybrid Drosophila melanogaster
19 CycG  
Two-hybrid Drosophila melanogaster
20 CG3225  
Two-hybrid Drosophila melanogaster
21 CG2540  
Two-hybrid Drosophila melanogaster
22 Crc  
Affinity Capture-MS Drosophila melanogaster
23 Tak1  
Affinity Capture-MS Drosophila melanogaster
24 CG11659  
Two-hybrid Drosophila melanogaster
25 key  
Affinity Capture-MS Drosophila melanogaster
26 Nlp  
Affinity Capture-MS Drosophila melanogaster
27 NHP2  
Two-hybrid Drosophila melanogaster
28 Fadd  
Affinity Capture-MS Drosophila melanogaster
29 imd  
Affinity Capture-MS Drosophila melanogaster
Two-hybrid Drosophila melanogaster
30 CG32820  
Two-hybrid Drosophila melanogaster
31 CG33017  
Two-hybrid Drosophila melanogaster
32 Ir56b  
Two-hybrid Drosophila melanogaster
33 PGRP-LE  
Affinity Capture-MS Drosophila melanogaster
34 CG32819  
Two-hybrid Drosophila melanogaster
35 CG17450  
Two-hybrid Drosophila melanogaster
36 ninaA  
Two-hybrid Drosophila melanogaster
37 PHDP  
Affinity Capture-MS Drosophila melanogaster
38 SmD3  
Two-hybrid Drosophila melanogaster
39 CG15109  
Two-hybrid Drosophila melanogaster
40 CG10265  
Affinity Capture-MS Drosophila melanogaster
41 CG6300  
Two-hybrid Drosophila melanogaster
42 Galphas  
Two-hybrid Drosophila melanogaster
43 Hmx  
Two-hybrid Drosophila melanogaster
44 Mdh2  
Affinity Capture-MS Drosophila melanogaster
45 Cpr64Aa  
Two-hybrid Drosophila melanogaster
46 Rel  
Affinity Capture-MS Drosophila melanogaster
47 dik  
Two-hybrid Drosophila melanogaster
48 CG13840  
Two-hybrid Drosophila melanogaster
49 ird5  
Affinity Capture-MS Drosophila melanogaster
50 RAF2  
Two-hybrid Drosophila melanogaster
51 PGRP-LC  
Affinity Capture-MS Drosophila melanogaster
52 CTPsyn  
Proximity Label-MS Drosophila melanogaster
53 esg  
Two-hybrid Drosophila melanogaster
54 Df31  
Affinity Capture-MS Drosophila melanogaster
55 regucalcin  
Affinity Capture-MS Drosophila melanogaster
56 AnxB10  
Affinity Capture-MS Drosophila melanogaster
57 Prosbeta3  
Two-hybrid Drosophila melanogaster
58 Tab2  
Affinity Capture-MS Drosophila melanogaster
59 BM-40-SPARC  
Two-hybrid Drosophila melanogaster
60 Nacalpha  
Affinity Capture-MS Drosophila melanogaster
61 Moe  
Affinity Capture-MS Drosophila melanogaster
62 mbl  
Two-hybrid Drosophila melanogaster
63 CG12934  
Two-hybrid Drosophila melanogaster
64 CG2116  
Two-hybrid Drosophila melanogaster
65 CG3918  
Two-hybrid Drosophila melanogaster
66 CG14089  
Two-hybrid Drosophila melanogaster
67 Abd-B  
Two-hybrid Drosophila melanogaster
68 RpL5 3355124
Two-hybrid Drosophila melanogaster
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